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Genome Scale Transcriptomics of Baculovirus-Insect Interactions
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia
* Author to whom correspondence should be addressed.
Received: 10 September 2013; in revised form: 28 October 2013 / Accepted: 4 November 2013 / Published: 12 November 2013
Abstract: Baculovirus-insect cell technologies are applied in the production of complex proteins, veterinary and human vaccines, gene delivery vectors‚ and biopesticides. Better understanding of how baculoviruses and insect cells interact would facilitate baculovirus-based production. While complete genomic sequences are available for over 58 baculovirus species, little insect genomic information is known. The release of the Bombyx mori and Plutella xylostella genomes, the accumulation of EST sequences for several Lepidopteran species, and especially the availability of two genome-scale analysis tools, namely oligonucleotide microarrays and next generation sequencing (NGS), have facilitated expression studies to generate a rich picture of insect gene responses to baculovirus infections. This review presents current knowledge on the interaction dynamics of the baculovirus-insect system‚ which is relatively well studied in relation to nucleocapsid transportation, apoptosis, and heat shock responses, but is still poorly understood regarding responses involved in pro-survival pathways, DNA damage pathways, protein degradation, translation, signaling pathways, RNAi pathways, and importantly metabolic pathways for energy, nucleotide and amino acid production. We discuss how the two genome-scale transcriptomic tools can be applied for studying such pathways and suggest that proteomics and metabolomics can produce complementary findings to transcriptomic studies.
Keywords: baculovirus; insect virus; virus-host interactions; microarray; RNA sequencing; next generation sequencing; genome scale; expression profile
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Cite This Article
MDPI and ACS Style
Nguyen, Q.; Nielsen, L.K.; Reid, S. Genome Scale Transcriptomics of Baculovirus-Insect Interactions. Viruses 2013, 5, 2721-2747.
Nguyen Q, Nielsen LK, Reid S. Genome Scale Transcriptomics of Baculovirus-Insect Interactions. Viruses. 2013; 5(11):2721-2747.
Nguyen, Quan; Nielsen, Lars K.; Reid, Steven. 2013. "Genome Scale Transcriptomics of Baculovirus-Insect Interactions." Viruses 5, no. 11: 2721-2747.