Viruses 2013, 5(11), 2721-2747; doi:10.3390/v5112721
Review

Genome Scale Transcriptomics of Baculovirus-Insect Interactions

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Received: 10 September 2013; in revised form: 28 October 2013 / Accepted: 4 November 2013 / Published: 12 November 2013
(This article belongs to the Section Insect Viruses)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Baculovirus-insect cell technologies are applied in the production of complex proteins, veterinary and human vaccines, gene delivery vectors‚ and biopesticides. Better understanding of how baculoviruses and insect cells interact would facilitate baculovirus-based production. While complete genomic sequences are available for over 58 baculovirus species, little insect genomic information is known. The release of the Bombyx mori and Plutella xylostella genomes, the accumulation of EST sequences for several Lepidopteran species, and especially the availability of two genome-scale analysis tools, namely oligonucleotide microarrays and next generation sequencing (NGS), have facilitated expression studies to generate a rich picture of insect gene responses to baculovirus infections. This review presents current knowledge on the interaction dynamics of the baculovirus-insect system‚ which is relatively well studied in relation to nucleocapsid transportation, apoptosis, and heat shock responses, but is still poorly understood regarding responses involved in pro-survival pathways, DNA damage pathways, protein degradation, translation, signaling pathways, RNAi pathways, and importantly metabolic pathways for energy, nucleotide and amino acid production. We discuss how the two genome-scale transcriptomic tools can be applied for studying such pathways and suggest that proteomics and metabolomics can produce complementary findings to transcriptomic studies.
Keywords: baculovirus; insect virus; virus-host interactions; microarray; RNA sequencing; next generation sequencing; genome scale; expression profile
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MDPI and ACS Style

Nguyen, Q.; Nielsen, L.K.; Reid, S. Genome Scale Transcriptomics of Baculovirus-Insect Interactions. Viruses 2013, 5, 2721-2747.

AMA Style

Nguyen Q, Nielsen LK, Reid S. Genome Scale Transcriptomics of Baculovirus-Insect Interactions. Viruses. 2013; 5(11):2721-2747.

Chicago/Turabian Style

Nguyen, Quan; Nielsen, Lars K.; Reid, Steven. 2013. "Genome Scale Transcriptomics of Baculovirus-Insect Interactions." Viruses 5, no. 11: 2721-2747.


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