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Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms
Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M2S), Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, 3001 Leuven, Belgium
Present address: Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, Universiteit Antwerpen, Universiteitsplein 1, S6.21, 2610 Antwerpen, Belgium.
* Author to whom correspondence should be addressed.
Received: 11 December 2012; in revised form: 3 January 2013 / Accepted: 9 January 2013 / Published: 11 January 2013
Abstract: In this study, three-day old mature biofilms of Escherichia coli were exposed once to either a temperate Shiga-toxin encoding phage (H-19B) or an obligatory lytic phage (T7), after which further dynamics in the biofilm were monitored. As such, it was found that a single dose of H-19B could rapidly lead to a near complete lysogenization of the biofilm, with a subsequent continuous release of infectious H-19B particles. On the other hand, a single dose of T7 rapidly led to resistance development in the biofilm population. Together, our data indicates a profound impact of phages on the dynamics within structured bacterial populations.
Keywords: Escherichia coli; biofilm; lysogenic conversion; resistance development; Shiga toxin; phage
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MDPI and ACS Style
Moons, P.; Faster, D.; Aertsen, A. Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms. Viruses 2013, 5, 150-161.
Moons P, Faster D, Aertsen A. Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms. Viruses. 2013; 5(1):150-161.
Moons, Pieter; Faster, David; Aertsen, Abram. 2013. "Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms." Viruses 5, no. 1: 150-161.