Next Article in Journal
Base Composition and Translational Selection are Insufficient to Explain Codon Usage Bias in Plant Viruses
Next Article in Special Issue
Phage Lambda P Protein: Trans-Activation, Inhibition Phenotypes and their Suppression
Previous Article in Journal
Approaches for Identification of HIV-1 Entry Inhibitors Targeting gp41 Pocket
Previous Article in Special Issue
Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage
Viruses 2013, 5(1), 150-161; doi:10.3390/v5010150
Communication

Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms

,
 and
*
Received: 11 December 2012 / Revised: 3 January 2013 / Accepted: 9 January 2013 / Published: 11 January 2013
(This article belongs to the Special Issue Recent Progress in Bacteriophage Research)
View Full-Text   |   Download PDF [378 KB, uploaded 12 May 2015]   |   Browse Figures

Abstract

In this study, three-day old mature biofilms of Escherichia coli were exposed once to either a temperate Shiga-toxin encoding phage (H-19B) or an obligatory lytic phage (T7), after which further dynamics in the biofilm were monitored. As such, it was found that a single dose of H-19B could rapidly lead to a near complete lysogenization of the biofilm, with a subsequent continuous release of infectious H-19B particles. On the other hand, a single dose of T7 rapidly led to resistance development in the biofilm population. Together, our data indicates a profound impact of phages on the dynamics within structured bacterial populations.
Keywords: Escherichia coli; biofilm; lysogenic conversion; resistance development; Shiga toxin; phage Escherichia coli; biofilm; lysogenic conversion; resistance development; Shiga toxin; phage
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Moons, P.; Faster, D.; Aertsen, A. Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms. Viruses 2013, 5, 150-161.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert