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Viruses 2013, 5(1), 150-161; doi:10.3390/v5010150
Communication

Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms

,
 and *
Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M2S), Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, 3001 Leuven, Belgium Present address: Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, Universiteit Antwerpen, Universiteitsplein 1, S6.21, 2610 Antwerpen, Belgium.
* Author to whom correspondence should be addressed.
Received: 11 December 2012 / Revised: 3 January 2013 / Accepted: 9 January 2013 / Published: 11 January 2013
(This article belongs to the Special Issue Recent Progress in Bacteriophage Research)
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Abstract

In this study, three-day old mature biofilms of Escherichia coli were exposed once to either a temperate Shiga-toxin encoding phage (H-19B) or an obligatory lytic phage (T7), after which further dynamics in the biofilm were monitored. As such, it was found that a single dose of H-19B could rapidly lead to a near complete lysogenization of the biofilm, with a subsequent continuous release of infectious H-19B particles. On the other hand, a single dose of T7 rapidly led to resistance development in the biofilm population. Together, our data indicates a profound impact of phages on the dynamics within structured bacterial populations.
Keywords: Escherichia coli; biofilm; lysogenic conversion; resistance development; Shiga toxin; phage Escherichia coli; biofilm; lysogenic conversion; resistance development; Shiga toxin; phage
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Moons, P.; Faster, D.; Aertsen, A. Lysogenic Conversion and Phage Resistance Development in Phage Exposed Escherichia coli Biofilms. Viruses 2013, 5, 150-161.

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