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PepGMV Rep-Protein Expression in Mammalian Cells
CA Ingeniería de Biosistemas, División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Santiago de Querétaro, Qro., 76010, México
División de Investigación y Posgrado, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias s/n, Juriquilla, Qro., 76230, México
* Author to whom correspondence should be addressed.
Received: 27 July 2012; in revised form: 6 September 2012 / Accepted: 6 September 2012 / Published: 24 September 2012
Abstract: The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals.
Keywords: Geminivirus; cell cycle; Rep protein
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Chapa-Oliver, A.M.; Mejía-Teniente, L.; García-Gasca, T.; Guevara-Gonzalez, R.G.; Torres-Pacheco, I. PepGMV Rep-Protein Expression in Mammalian Cells. Viruses 2012, 4, 1792-1801.
Chapa-Oliver AM, Mejía-Teniente L, García-Gasca T, Guevara-Gonzalez RG, Torres-Pacheco I. PepGMV Rep-Protein Expression in Mammalian Cells. Viruses. 2012; 4(9):1792-1801.
Chapa-Oliver, Angela María; Mejía-Teniente, Laura; García-Gasca, Teresa; Guevara-Gonzalez, Ramon Gerardo; Torres-Pacheco, Irineo. 2012. "PepGMV Rep-Protein Expression in Mammalian Cells." Viruses 4, no. 9: 1792-1801.