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Viruses 2012, 4(9), 1792-1801; doi:10.3390/v4091792
Article

PepGMV Rep-Protein Expression in Mammalian Cells

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Received: 27 July 2012; in revised form: 6 September 2012 / Accepted: 6 September 2012 / Published: 24 September 2012
(This article belongs to the Special Issue Plant Viruses)
View Full-Text   |   Download PDF [613 KB, uploaded 24 September 2012]
Abstract: The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals.
Keywords: Geminivirus; cell cycle; Rep protein Geminivirus; cell cycle; Rep protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chapa-Oliver, A.M.; Mejía-Teniente, L.; García-Gasca, T.; Guevara-Gonzalez, R.G.; Torres-Pacheco, I. PepGMV Rep-Protein Expression in Mammalian Cells. Viruses 2012, 4, 1792-1801.

AMA Style

Chapa-Oliver AM, Mejía-Teniente L, García-Gasca T, Guevara-Gonzalez RG, Torres-Pacheco I. PepGMV Rep-Protein Expression in Mammalian Cells. Viruses. 2012; 4(9):1792-1801.

Chicago/Turabian Style

Chapa-Oliver, Angela María; Mejía-Teniente, Laura; García-Gasca, Teresa; Guevara-Gonzalez, Ramon Gerardo; Torres-Pacheco, Irineo. 2012. "PepGMV Rep-Protein Expression in Mammalian Cells." Viruses 4, no. 9: 1792-1801.


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