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Viruses 2012, 4(4), 539-556; doi:10.3390/v4040539

Daphne Genkwa Sieb. et Zucc. Water-Soluble Extracts Act on Enterovirus 71 by Inhibiting Viral Entry

1,†
,
2,3,†
and
1,4,†,*
1
Department of Biochemistry, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
2
Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
3
Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
4
Research Center for Emerging Viral Infections, Chang Gung University, Kweishan, Taoyuan 333, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 7 March 2012 / Revised: 26 March 2012 / Accepted: 3 April 2012 / Published: 11 April 2012
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Abstract

Dried flowers of Daphne genkwa Sieb. et Zucc. (Thymelaeaceae) are a Chinese herbal medicine used as an abortifacient with purgative, diuretic and anti-inflammatory activities. However, the activity of this medicine against enteroviral infections has not been investigated. The water-extract of dried buds of D. genkwa Sieb. et Zucc. (DGFW) was examined against various strains of enterovirus 71 (EV71) by neutralization assay, and its initial mode of action was characterized by time-of-addition assay followed by attachment and penetration assays. Pretreatment of DGFW with virus abolished viral replication, indicating that DGFW inhibits EV71 by targeting the virus. GFW exerts its anti-EV71 effects by inhibiting viral entry without producing cytotoxic side effects and thus provides a potential agent for antiviral chemotherapeutics.
Keywords: attachment assay; cytopathic effect; enterovirus 71; Daphne Genkwa Sieb. et Zucc.; penetration assay; yuanhua attachment assay; cytopathic effect; enterovirus 71; Daphne Genkwa Sieb. et Zucc.; penetration assay; yuanhua
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chang, C.-W.; Leu, Y.-L.; Horng, J.-T. Daphne Genkwa Sieb. et Zucc. Water-Soluble Extracts Act on Enterovirus 71 by Inhibiting Viral Entry. Viruses 2012, 4, 539-556.

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