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Phosphoinositides in the Hepatitis C Virus Life Cycle
Division of Biological Sciences, University of California, San Diego. 9500 Gilman Dr., San Diego, CA, 92093, USA
Division of Infectious Diseases, University of California, San Diego. 9500 Gilman Dr., San Diego, CA, 92093, USA
* Author to whom correspondence should be addressed.
Received: 4 September 2012; in revised form: 5 October 2012 / Accepted: 9 October 2012 / Published: 19 October 2012
Abstract: Eukaryotes possess seven different phosphoinositides (PIPs) that help form the unique signatures of various intracellular membranes. PIPs serve as docking sites for the recruitment of specific proteins to mediate membrane alterations and integrate various signaling cascades. The spatio-temporal regulation of PI kinases and phosphatases generates distinct intracellular hubs of PIP signaling. Hepatitis C virus (HCV), like other plus-strand RNA viruses, promotes the rearrangement of intracellular membranes to assemble viral replication complexes. HCV stimulates enrichment of phosphatidylinositol 4-phosphate (PI4P) pools near endoplasmic reticulum (ER) sites by activating PI4KIIIα, the kinase responsible for generation of ER-specific PI4P pools. Inhibition of PI4KIIIα abrogates HCV replication. PI4P, the most abundant phosphoinositide, predominantly localizes to the Golgi and plays central roles in Golgi secretory functions by recruiting effector proteins involved in transport vesicle generation. The PI4P effector proteins also include the lipid-transfer and structural proteins such as ceramide transfer protein (CERT), oxysterol binding protein (OSBP) and Golgi phosphoprotein 3 (GOLPH3) that help maintain Golgi-membrane composition and structure. Depletion of Golgi-specific PI4P pools by silencing PI4KIIIβ, expression of dominant negative CERT and OSBP mutants, or silencing GOLPH3 perturb HCV secretion. In this review we highlight the role of PIPs and specifically PI4P in the HCV life cycle.
Keywords: HCV; hepatitis C; PI4P; phosphoinositides; PI4KIIIα; PI4KIIIβ
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Cite This Article
MDPI and ACS Style
Bishé, B.; Syed, G.; Siddiqui, A. Phosphoinositides in the Hepatitis C Virus Life Cycle. Viruses 2012, 4, 2340-2358.
Bishé B, Syed G, Siddiqui A. Phosphoinositides in the Hepatitis C Virus Life Cycle. Viruses. 2012; 4(10):2340-2358.
Bishé, Bryan; Syed, Gulam; Siddiqui, Aleem. 2012. "Phosphoinositides in the Hepatitis C Virus Life Cycle." Viruses 4, no. 10: 2340-2358.