A correction was published on 20 March 2013, see Viruses 2013, 5(3), 901.

Viruses 2012, 4(10), 1844-1864; doi:10.3390/v4101844
Article

miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction

1 Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, 733 N. Broadway, Edward D. Miller Research Building, Baltimore, MD 21205,USA 2 Department of Neurology, The Johns Hopkins University School of Medicine, 733 N. Broadway, Edward D. Miller Research Building, Baltimore, MD 21205, USA 3 Department of Pathology, The Johns Hopkins University School of Medicine, 733 N. Broadway, Edward D. Miller Research Building, Baltimore, MD 21205,USA
* Author to whom correspondence should be addressed.
Received: 25 July 2012; in revised form: 8 September 2012 / Accepted: 11 September 2012 / Published: 25 September 2012
(This article belongs to the Special Issue Viruses and miRNAs)
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Abstract: Long-lived HIV-1 reservoirs include tissue macrophages. Monocyte-derived macrophages are more susceptible to infection and more permissive to HIV-1 replication than monocytes for reasons that may include the effects of different populations of miRNAs in these two cell classes. Specifically, miRs-28-3p, -150, -223, -198, and -382 exert direct or indirect negative effects on HIV-1 and are reportedly downmodulated during monocyte-to-macrophage differentiation. Here, new experimental results are presented along with reviews and analysis of published studies and publicly available datasets, supporting a broader role of miRNAs in HIV-1 restriction than would be suggested by a simple and uniform downregulation of anti-HIV miRNAs during monocyte-to-macrophage differentiation. Although miR-223 is downregulated in macrophages, other putatively antiviral miRNAs are more abundant in macrophages than in monocytes or are rare and/or variably present in both cell classes. Our analyses point to the need for further studies to determine miRNA profiles of monocytes and macrophages, including classic and newly identified subpopulations; examine the sensitivity of miRNA profiling to cell isolation and differentiation protocols; and characterize rigorously the antiviral effects of previously reported and novel predicted miRNA-HIV-1 interactions in cell-specific contexts.
Keywords: microRNA; HIV-1; monocyte; macrophage; profiling; antiviral

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MDPI and ACS Style

Sisk, J.M.; Clements, J.E.; Witwer, K.W. miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction. Viruses 2012, 4, 1844-1864.

AMA Style

Sisk JM, Clements JE, Witwer KW. miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction. Viruses. 2012; 4(10):1844-1864.

Chicago/Turabian Style

Sisk, Jeanne M.; Clements, Janice E.; Witwer, Kenneth W. 2012. "miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction." Viruses 4, no. 10: 1844-1864.

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