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Viruses 2011, 3(8), 1485-1500; doi:10.3390/v3081485
Article

Direct Inhibition of RNAse T2 Expression by the HTLV-1 Viral Protein Tax

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Received: 4 August 2011; Accepted: 10 August 2011 / Published: 18 August 2011
(This article belongs to the Special Issue Recent Developments in HTLV Research)
Download PDF [437 KB, uploaded 18 August 2011]
Abstract: Adult T-cell leukemia (ATL) is one of the primary diseases caused by Human T-cell Leukemia Virus type 1 (HTLV-1) infection. The virally-encoded Tax protein is believed to initiate early events in the development of this disease, as it is able to promote immortalization of T-cells and transformation of other cell types. These processes may be aided by the ability of the viral protein to directly deregulate expression of specific cellular genes through interactions with numerous transcriptional regulators. To identify gene promoters where Tax is localized, we isolated Tax-DNA complexes from an HTLV-1-infected T-cell line through a chromatin immunoprecipitation (ChIP) assay and used the DNA to probe a CpG island microarray. A site within the RNASET2 gene was found to be occupied by Tax. Real-time PCR analysis confirmed this result, and transient expression of Tax in uninfected cells led to the recruitment of the viral protein to the promoter. This event correlated with a decrease in the level of RNase T2 mRNA and protein, suggesting that Tax represses expression of this gene. Loss of RNase T2 expression occurs in certain hematological malignancies and other forms of cancer, and RNase T2 was recently reported to function as a tumor suppressor. Consequently, a reduction in the level of RNase T2 by Tax may play a role in ATL development.
Keywords: HTLV-1; Tax; RNase T2; repression; transcription HTLV-1; Tax; RNase T2; repression; transcription
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Polakowski, N.; Han, H.; Lemasson, I. Direct Inhibition of RNAse T2 Expression by the HTLV-1 Viral Protein Tax. Viruses 2011, 3, 1485-1500.

AMA Style

Polakowski N, Han H, Lemasson I. Direct Inhibition of RNAse T2 Expression by the HTLV-1 Viral Protein Tax. Viruses. 2011; 3(8):1485-1500.

Chicago/Turabian Style

Polakowski, Nicholas; Han, Hongjin; Lemasson, Isabelle. 2011. "Direct Inhibition of RNAse T2 Expression by the HTLV-1 Viral Protein Tax." Viruses 3, no. 8: 1485-1500.


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