Viruses 2011, 3(10), 1959-1985; doi:10.3390/v3101959
Review

The Molecular Biology of Frog Virus 3 and other Iridoviruses Infecting Cold-Blooded Vertebrates

1,* email, 1 and 2
Received: 30 August 2011; in revised form: 27 September 2011 / Accepted: 27 September 2011 / Published: 20 October 2011
(This article belongs to the Special Issue Viruses Infecting Fish, Amphibians, and Reptiles)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Frog virus 3 (FV3) is the best characterized member of the family Iridoviridae. FV3 study has provided insights into the replication of other family members, and has served as a model of viral transcription, genome replication, and virus-mediated host-shutoff. Although the broad outlines of FV3 replication have been elucidated, the precise roles of most viral proteins remain unknown. Current studies using knock down (KD) mediated by antisense morpholino oligonucleotides (asMO) and small, interfering RNAs (siRNA), knock out (KO) following replacement of the targeted gene with a selectable marker by homologous recombination, ectopic viral gene expression, and recombinant viral proteins have enabled researchers to systematically ascertain replicative- and virulence-related gene functions. In addition, the application of molecular tools to ecological studies is providing novel ways for field biologists to identify potential pathogens, quantify infections, and trace the evolution of ecologically important viral species. In this review, we summarize current studies using not only FV3, but also other iridoviruses infecting ectotherms. As described below, general principles ascertained using FV3 served as a model for the family, and studies utilizing other ranaviruses and megalocytiviruses have confirmed and extended our understanding of iridovirus replication. Collectively, these and future efforts will elucidate molecular events in viral replication, intrinsic and extrinsic factors that contribute to disease outbreaks, and the role of the host immune system in protection from disease.
Keywords: ranavirus; Iridoviridae; virus replication; viral gene function; ectothermic vertebrates; antisense morpholino oligonucleotides; knock out mutants
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MDPI and ACS Style

Chinchar, V.G.; Yu, K.H.; Jancovich, J.K. The Molecular Biology of Frog Virus 3 and other Iridoviruses Infecting Cold-Blooded Vertebrates. Viruses 2011, 3, 1959-1985.

AMA Style

Chinchar VG, Yu KH, Jancovich JK. The Molecular Biology of Frog Virus 3 and other Iridoviruses Infecting Cold-Blooded Vertebrates. Viruses. 2011; 3(10):1959-1985.

Chicago/Turabian Style

Chinchar, V. Gregory; Yu, Kwang H.; Jancovich, James K. 2011. "The Molecular Biology of Frog Virus 3 and other Iridoviruses Infecting Cold-Blooded Vertebrates." Viruses 3, no. 10: 1959-1985.

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