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Viruses 2018, 10(9), 482; https://doi.org/10.3390/v10090482

Characterization of vB_Kpn_F48, a Newly Discovered Lytic Bacteriophage for Klebsiella pneumoniae of Sequence Type 101

1
Department of Biology, University of Rome “Tor Vergata”, 00133 Rome, Italy
2
Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy
3
Center for Integrative Biology, CIBIO, University of Trento, 38122 Trento, Italy
4
InBioS—Center for Protein Engineering, Department of Life Sciences, University of Liège, Sart-Tilman, 4000 Liège, Belgium
5
Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy
6
Department of Life Sciences, University of Siena, 53100 Siena, Italy
7
Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, 50134 Florence, Italy
*
Author to whom correspondence should be addressed.
Received: 22 August 2018 / Revised: 5 September 2018 / Accepted: 7 September 2018 / Published: 9 September 2018
(This article belongs to the Section Bacterial Viruses)
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Abstract

Resistance to carbapenems in Enterobacteriaceae, including Klebsiella pneumoniae, represents a major clinical problem given the lack of effective alternative antibiotics. Bacteriophages could provide a valuable tool to control the dissemination of antibiotic resistant isolates, for the decolonization of colonized individuals and for treatment purposes. In this work, we have characterized a lytic bacteriophage, named vB_Kpn_F48, specific for K. pneumoniae isolates belonging to clonal group 101. Phage vB_Kpn_F48 was classified as a member of Myoviridae, order Caudovirales, on the basis of transmission electron microscopy analysis. Physiological characterization demonstrated that vB_Kpn_F48 showed a narrow host range, a short latent period, a low burst size and it is highly stable to both temperature and pH variations. High throughput sequencing and bioinformatics analysis revealed that the phage is characterized by a 171 Kb dsDNA genome that lacks genes undesirable for a therapeutic perspective such integrases, antibiotic resistance genes and toxin encoding genes. Phylogenetic analysis suggests that vB_Kpn_F48 is a T4-like bacteriophage which belongs to a novel genus within the Tevenvirinae subfamily, which we tentatively named “F48virus”. Considering the narrow host range, the genomic features and overall physiological parameters phage vB_Kpn_F48 could be a promising candidate to be used alone or in cocktails for phage therapy applications. View Full-Text
Keywords: bacteriophage; Tevenvirinae; Klebsiella pneumoniae carbapenemase (KPC); K. pneumoniae; sequence type 101; Klebsiella pneumoniae ST101 bacteriophage; Tevenvirinae; Klebsiella pneumoniae carbapenemase (KPC); K. pneumoniae; sequence type 101; Klebsiella pneumoniae ST101
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ciacci, N.; D’Andrea, M.M.; Marmo, P.; Demattè, E.; Amisano, F.; Pilato, V.D.; Fraziano, M.; Lupetti, P.; Rossolini, G.M.; Thaller, M.C. Characterization of vB_Kpn_F48, a Newly Discovered Lytic Bacteriophage for Klebsiella pneumoniae of Sequence Type 101. Viruses 2018, 10, 482.

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