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Viruses 2018, 10(9), 449; https://doi.org/10.3390/v10090449

The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA

1
Department of Entomology, Animal Pests and Biotechnology, Institute of Plant Protection—National Research Institute, 20 Władysława Węgorka Street, 60-318 Poznań, Poland
2
Institute of Bioorganic Chemistry, Polish Academy of Sciences, 12/14 Noskowskiego Street, 61-704 Poznań, Poland
3
Institute of Computing Science, Faculty of Computing Science, Poznań University of Technology, 2 Piotrowo Street, 60-965 Poznań, Poland
4
Department of Mathematical and Statistical Methods, University of Life Sciences in Poznań, Wojska Polskiego 28 Street, 60-637 Poznań, Poland
5
Department of Virology, Institute of Plant Protection—National Research Institute, 20 Władysława Węgorka Street, 60-318 Poznań, Poland
*
Author to whom correspondence should be addressed.
Received: 17 July 2018 / Revised: 2 August 2018 / Accepted: 22 August 2018 / Published: 23 August 2018
(This article belongs to the Special Issue Plant-Virus Interactions)
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Abstract

Peanut stunt virus (PSV) is a widespread pathogen infecting legumes. The PSV strains are classified into four subgroups and some are defined by the association of satellite RNAs (satRNAs). In the case of PSV, the presence of satRNAs alters the symptoms of disease in infected plants. In this study, we elucidated the plant response to PSV-G strain, which occurs in natural conditions without satRNA. However, it was found that it might easily acquire satRNA, which exacerbated pathogenesis in Nicotiana benthamiana. To explain the mechanisms underlying PSV infection and symptoms exacerbation caused by satRNA, we carried out transcriptome profiling of N. benthamiana challenged by PSV-G and satRNA using species-specific microarrays. Co-infection of plants with PSV-G + satRNA increased the number of identified differentially expressed genes (DEGs) compared with the number identified in PSV-G-infected plants. In both treatments, the majority of up-regulated DEGs were engaged in translation, ribosome biogenesis, RNA metabolism, and response to stimuli, while the down-regulated DEGs were required for photosynthesis. The presence of satRNA in PSV-G-infected plants caused different trends in expression of DEGs associated with phosphorylation, ATP binding, and plasma membrane. View Full-Text
Keywords: plant–virus interaction; satellite RNA; transcriptomics; leaf transcriptome; plant defense; stress response; phosphorylation; posttranscriptional gene silencing (PTGS) plant–virus interaction; satellite RNA; transcriptomics; leaf transcriptome; plant defense; stress response; phosphorylation; posttranscriptional gene silencing (PTGS)
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Obrępalska-Stęplowska, A.; Zmienko, A.; Wrzesińska, B.; Goralski, M.; Figlerowicz, M.; Zyprych-Walczak, J.; Siatkowski, I.; Pospieszny, H. The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA. Viruses 2018, 10, 449.

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