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Viruses 2018, 10(8), 441; https://doi.org/10.3390/v10080441

Rapidity of Genomic Adaptations to Prasinovirus Infection in a Marine Microalga

1
Integrative Biology of Marine Organisms Laboratory (BIOM), CNRS UMR7232, 66650 Banuyls-sur-Mer, France
2
Sorbonne University, OOB, Avenue de Pierre Fabre, 66650 Banyuls-sur-Mer, France
*
Author to whom correspondence should be addressed.
Received: 31 July 2018 / Revised: 16 August 2018 / Accepted: 17 August 2018 / Published: 19 August 2018
(This article belongs to the Special Issue Algae Virus)
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Abstract

Prasinoviruses are large dsDNA viruses commonly found in aquatic systems worldwide, where they can infect and lyse unicellular prasinophyte algae such as Ostreococcus. Host susceptibility is virus strain-specific, but resistance of susceptible Ostreococcus tauri strains to a virulent virus arises frequently. In clonal resistant lines that re-grow, viruses are usually present for many generations, and genes clustered on chromosome 19 show physical rearrangements and differential expression. Here, we investigated changes occurring during the first two weeks after inoculation of the prasinovirus OtV5. By serial dilutions of cultures at the time of inoculation, we estimated the frequency of resistant cells arising in virus-challenged O. tauri cultures to be 10−3–10−4 of the inoculated population. Re-growing resistant cells were detectable by flow cytometry 3 days post-inoculation (dpi), visible re-greening of cultures occurred by 6 dpi, and karyotypic changes were visually detectable at 8 dpi. Resistant cell lines showed a modified spectrum of host-virus specificities and much lower levels of OtV5 adsorption. View Full-Text
Keywords: Ostreococcus tauri; Mamiellophyceae; Phycodnaviridae; resistance; karyotype; rearrangement; chromosome; specificity; host range; adsorption Ostreococcus tauri; Mamiellophyceae; Phycodnaviridae; resistance; karyotype; rearrangement; chromosome; specificity; host range; adsorption
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Yau, S.; Caravello, G.; Fonvieille, N.; Desgranges, É.; Moreau, H.; Grimsley, N. Rapidity of Genomic Adaptations to Prasinovirus Infection in a Marine Microalga. Viruses 2018, 10, 441.

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