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Viruses 2018, 10(7), 371; https://doi.org/10.3390/v10070371

Conserved Active-Site Residues Associated with OAS Enzyme Activity and Ubiquitin-Like Domains Are Not Required for the Antiviral Activity of goOASL Protein against Avian Tembusu Virus

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1
Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2
Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
3
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 16 May 2018 / Revised: 27 June 2018 / Accepted: 10 July 2018 / Published: 15 July 2018
(This article belongs to the Section Antivirals & Vaccines)
View Full-Text   |   Download PDF [1805 KB, uploaded 15 July 2018]   |  

Abstract

Interferon (IFN)-induced 2′-5′-oligoadenylate synthetase (OAS) proteins exhibit an extensive and efficient antiviral effect against flavivirus infection in mammals and birds. Only the 2′-5′-oligoadenylate synthetase-like (OASL) gene has been identified thus far in birds, except for ostrich, which has both OAS1 and OASL genes. In this study, we first investigated the antiviral activity of goose OASL (goOASL) protein against a duck-origin Tembusu virus (DTMUV) in duck embryo fibroblast cells (DEFs). To investigate the relationship of conserved amino acids that are related to OAS enzyme activity and ubiquitin-like (UBL) domains with the antiviral activity of goOASL, a series of mutant goOASL plasmids was constructed, including goOASL-S64C/D76E/D78E/D144T, goOASL∆UBLs and goOASL∆UBLs-S64C/D76E/D78E/D144T. Interestingly, all these mutant proteins significantly inhibited the replication of DTMUV in DEFs in a dose-dependent manner. Immunofluorescence analysis showed that the goOASL, goOASL-S64C/D76E/D78E/D144T, goOASL∆UBLs and goOASL∆UBLs-S64C/D76E/D78E/D144T proteins were located not only in the cytoplasm where DTMUV replicates but also in the nucleus of DEFs. However, the goOASL and goOASL mutant proteins were mainly colocalized with DTMUV in the cytoplasm of infected cells. Our data indicated that goOASL could significantly inhibit DTMUV replication in vitro, while the active-site residues S64, D76, D78 and D144, which were associated with OAS enzyme activity, the UBL domains were not required for the antiviral activity of goOASL protein. View Full-Text
Keywords: goose; 2′-5′-oligoadenylate synthetase-like; duck-origin Tembusu virus; antiviral activity; 2′-5′-oligoadenylate synthetase enzyme activity; ubiquitin-like domains goose; 2′-5′-oligoadenylate synthetase-like; duck-origin Tembusu virus; antiviral activity; 2′-5′-oligoadenylate synthetase enzyme activity; ubiquitin-like domains
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Chen, S.; Yang, C.; Zhang, J.; Wu, Z.; Wang, M.; Jia, R.; Zhu, D.; Liu, M.; Yang, Q.; Wu, Y.; Zhao, X.; Zhang, S.; Liu, Y.; Zhang, L.; Yu, Y.; You, Y.; Cheng, A. Conserved Active-Site Residues Associated with OAS Enzyme Activity and Ubiquitin-Like Domains Are Not Required for the Antiviral Activity of goOASL Protein against Avian Tembusu Virus. Viruses 2018, 10, 371.

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