Next Article in Journal
Small RNA NGS Revealed the Presence of Cherry Virus A and Little Cherry Virus 1 on Apricots in Hungary
Previous Article in Journal
Antiviral Effects of Clinically-Relevant Interferon-α and Ribavirin Regimens against Dengue Virus in the Hollow Fiber Infection Model (HFIM)
Previous Article in Special Issue
Non-Primate Lentiviral Vectors and Their Applications in Gene Therapy for Ocular Disorders
Article Menu

Export Article

Open AccessArticle
Viruses 2018, 10(6), 315; https://doi.org/10.3390/v10060315

Genetic Characterization and Phylogenetic Analysis of Small Ruminant Lentiviruses Detected in Spanish Assaf Sheep with Different Mammary Lesions

1
Pathological Anatomy Section, Animal Health Department, School of Veterinary Medicine, University of Leon, via Profesor Pedro Carmenes s/n Campus de Vegazana, 24071 León, Spain
2
School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica (MC), Italy
3
Micros Veterinaria, INDEGSAL, via Profesor Pedro Carmenes s/n Campus de Vegazana, 24071 León, Spain
*
Author to whom correspondence should be addressed.
Received: 7 April 2018 / Revised: 30 May 2018 / Accepted: 7 June 2018 / Published: 9 June 2018
(This article belongs to the Special Issue Nonprimate Lentivirus)
View Full-Text   |   Download PDF [2473 KB, uploaded 11 June 2018]   |  

Abstract

Small Ruminant Lentiviruses (SRLVs) are widespread in many countries and cause economically relevant, slow, and persistent diseases in sheep and goats. Monitoring the genetic diversity of SRLVs is useful to improve the diagnostic tools used in the eradication programs. In this study, SRLVs detected in Spanish Assaf sheep with different grades of lymphoproliferative mastitis were sequenced. Genetic characterization showed that most samples belonged to type A and were closer to Spanish SRLV isolates previously classified as A2/A3. Four samples belonged to subtype B2 and showed higher homology with Italian B2 strains than with Spanish B2 isolates. Amino acid sequences of immuno-dominant epitopes in the gag region were very conserved while more alterations were found in the LTR sequences. No significant correlations were found between grades of mastitis and alterations in the sequences although samples with similar histological features were phylogenetically closer to each other. Broader genetic characterization surveys in samples with different grades of SRLV-lesions are required for evaluating potential correlations between SRLV sequences and the severity of diseases. View Full-Text
Keywords: sheep; Small Ruminant Lentivirus; genetic characterization; udder; histopathology sheep; Small Ruminant Lentivirus; genetic characterization; udder; histopathology
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Gayo, E.; Cuteri, V.; Polledo, L.; Rossi, G.; García Marín, J.F.; Preziuso, S. Genetic Characterization and Phylogenetic Analysis of Small Ruminant Lentiviruses Detected in Spanish Assaf Sheep with Different Mammary Lesions. Viruses 2018, 10, 315.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top