Next Article in Journal
Characterization of an N-Terminal Non-Core Domain of RAG1 Gene Disrupted Syrian Hamster Model Generated by CRISPR Cas9
Previous Article in Journal
A CRISPR-Cas9-Based Toolkit for Fast and Precise In Vivo Genetic Engineering of Bacillus subtilis Phages
Previous Article in Special Issue
Does BCA3 Play a Role in the HIV-1 Replication Cycle?
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessCommunication
Viruses 2018, 10(5), 242; https://doi.org/10.3390/v10050242

Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting

1
The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UK
2
Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases, Binzhou 256600, Shandong, China
*
Authors to whom correspondence should be addressed.
Received: 27 March 2018 / Revised: 30 April 2018 / Accepted: 3 May 2018 / Published: 5 May 2018
View Full-Text   |   Download PDF [2475 KB, uploaded 5 May 2018]   |  

Abstract

Several studies have shown that microRNA-targeting is an effective strategy for the selective control of tissue-tropism and pathogenesis of both DNA and RNA viruses. However, the exploitation of microRNA-targeting for the inhibition of transformation by oncogenic viruses has not been studied. The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family of transcription factors capable of transforming primary chicken spleen and bone marrow cells. Here, by engineering the target sequence of endogenous microRNA miR-142 downstream of the v-rel gene in a Replication-Competent ALV (avian leukosis virus) long terminal repeat (LTR) with a splice acceptor (RCAS) vector and using a v-rel-induced transformation model of chicken embryonic splenocyte cultures, we show that hematopoietic-specific miR-142 can inhibit the v-rel-induced transformation, and that this inhibition effect is due to the silencing of v-rel expression. The data supports the idea that microRNA-targeting can be used to inhibit viral oncogene-induced oncogenesis. View Full-Text
Keywords: microRNA-targeting; inhibition; v-rel-induced transformation; hematopoietic specific miRNA microRNA-targeting; inhibition; v-rel-induced transformation; hematopoietic specific miRNA
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Yao, Y.; Zhang, Y.; Tang, N.; Pedrera, M.; Shen, Z.; Nair, V. Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting. Viruses 2018, 10, 242.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top