Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting
AbstractSeveral studies have shown that microRNA-targeting is an effective strategy for the selective control of tissue-tropism and pathogenesis of both DNA and RNA viruses. However, the exploitation of microRNA-targeting for the inhibition of transformation by oncogenic viruses has not been studied. The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family of transcription factors capable of transforming primary chicken spleen and bone marrow cells. Here, by engineering the target sequence of endogenous microRNA miR-142 downstream of the v-rel gene in a Replication-Competent ALV (avian leukosis virus) long terminal repeat (LTR) with a splice acceptor (RCAS) vector and using a v-rel-induced transformation model of chicken embryonic splenocyte cultures, we show that hematopoietic-specific miR-142 can inhibit the v-rel-induced transformation, and that this inhibition effect is due to the silencing of v-rel expression. The data supports the idea that microRNA-targeting can be used to inhibit viral oncogene-induced oncogenesis. View Full-Text
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Yao, Y.; Zhang, Y.; Tang, N.; Pedrera, M.; Shen, Z.; Nair, V. Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting. Viruses 2018, 10, 242.
Yao Y, Zhang Y, Tang N, Pedrera M, Shen Z, Nair V. Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting. Viruses. 2018; 10(5):242.Chicago/Turabian Style
Yao, Yongxiu; Zhang, Yaoyao; Tang, Na; Pedrera, Miriam; Shen, Zhiqiang; Nair, Venugopal. 2018. "Inhibition of v-rel-Induced Oncogenesis through microRNA Targeting." Viruses 10, no. 5: 242.
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