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Viruses 2018, 10(4), 212; https://doi.org/10.3390/v10040212

Does BCA3 Play a Role in the HIV-1 Replication Cycle?

1
Department of Biotechnology, University of Chemistry and Technology, Technická 5, 16628 Prague, Czech Republic
2
Department of Biochemistry and Microbiology, University of Chemistry and Technology, Technická 3, 16628 Prague, Czech Republic
3
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám 2, 16610 Prague, Czech Republic
*
Authors to whom correspondence should be addressed.
Received: 20 February 2018 / Revised: 5 April 2018 / Accepted: 18 April 2018 / Published: 20 April 2018
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Abstract

The cellular role of breast carcinoma-associated protein (BCA3), also known as A-kinase-interacting protein 1 (AKIP-1), is not fully understood. Recently, we reported that full-length, but not C-terminally truncated, BCA3 is incorporated into virions of Mason-Pfizer monkey virus, and that BCA3 enhances HIV-1 protease-induced apoptosis. In the present study, we report that BCA3 is associated with purified and subtilisin-treated HIV particles. Using a combination of immune-based methods and confocal microscopy, we show that the C-terminus of BCA3 is required for packaging into HIV-1 particles. However, we were unable to identify an HIV-1 binding domain for BCA3, and we did not observe any effect of incorporated BCA3 on HIV-1 infectivity. Interestingly, the BCA3 C-terminus was previously identified as a binding site for the catalytic subunit of protein kinase A (PKAc), a cellular protein that is specifically packaged into HIV-1 particles. Based on our analysis of PKAc–BCA3 interactions, we suggest that BCA3 incorporation into HIV-1 particles is mediated by its ability to interact with PKAc. View Full-Text
Keywords: HIV-1; BCA3; AKIP-1; M-PMV; virus incorporation; PKAc HIV-1; BCA3; AKIP-1; M-PMV; virus incorporation; PKAc
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Rumlová, M.; Křížová, I.; Zelenka, J.; Weber, J.; Ruml, T. Does BCA3 Play a Role in the HIV-1 Replication Cycle? Viruses 2018, 10, 212.

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