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Viruses 2018, 10(2), 78; https://doi.org/10.3390/v10020078

First Diagnosed Case of Camelpox Virus in Israel

1
Division of Virology, Kimron Veterinary Institute, P.O. Box 12, Beit Dagan 50250, Israel
2
Department of Infectious Diseases, IIBR P.O. Box 19, Ness Ziona 74100, Israel
3
Negev Veterinary Bureau, Israeli Veterinary Services, Binyamin Ben Asa 1, Be′er Sheba 84102, Israel
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 10 January 2018 / Revised: 6 February 2018 / Accepted: 12 February 2018 / Published: 13 February 2018
(This article belongs to the Section Animal Viruses)
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Abstract

An outbreak of a disease in camels with skin lesions was reported in Israel during 2016. To identify the etiological agent of this illness, we employed a multidisciplinary diagnostic approach. Transmission electron microscopy (TEM) analysis of lesion material revealed the presence of an orthopox-like virus, based on its characteristic brick shape. The virus from the skin lesions successfully infected chorioallantoic membranes and induced cytopathic effect in Vero cells, which were subsequently positively stained by an orthopox-specific antibody. The definite identification of the virus was accomplished by two independent qPCR, one of which was developed in this study, followed by sequencing of several regions of the viral genome. The qPCR and sequencing results confirmed the presence of camelpox virus (CMLV), and indicated that it is different from the previously annotated CMLV sequence available from GenBank. This is the first reported case of CMLV in Israel, and the first description of the isolated CMLV subtype. View Full-Text
Keywords: orthopoxvirus; camelpox virus; transmission electron microscopy; PCR; Immunofluorescence assay (IFA); chorioallantoic membrane orthopoxvirus; camelpox virus; transmission electron microscopy; PCR; Immunofluorescence assay (IFA); chorioallantoic membrane
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Erster, O.; Melamed, S.; Paran, N.; Weiss, S.; Khinich, Y.; Gelman, B.; Solomony, A.; Laskar-Levy, O. First Diagnosed Case of Camelpox Virus in Israel. Viruses 2018, 10, 78.

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