Next Article in Journal
Preparation and Application of Water-in-Oil Emulsions Stabilized by Modified Graphene Oxide
Next Article in Special Issue
Protein/Peptide Aggregation and Amyloidosis on Biointerfaces
Previous Article in Journal
Combined Effects of Non-Conforming Fly Ash and Recycled Masonry Aggregates on Mortar Properties
Previous Article in Special Issue
Rapid Assay to Assess Bacterial Adhesion on Textiles
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Materials 2016, 9(9), 730; doi:10.3390/ma9090730

Screening of Osteogenic-Enhancing Short Peptides from BMPs for Biomimetic Material Applications

1
Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan
2
Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
3
Biomaterials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Ibaraki, Japan
4
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Aichi, Japan
5
Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, 65 Turumai-cho, Showa-ku, Nagoya 466-8550, Aichi, Japan
6
Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 65 Turumai-cho, Showa-ku, Nagoya 466-8550, Aichi, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Qun Ren
Received: 15 July 2016 / Revised: 17 August 2016 / Accepted: 19 August 2016 / Published: 25 August 2016
(This article belongs to the Special Issue Advances in Biointerfaces)
View Full-Text   |   Download PDF [3790 KB, uploaded 25 August 2016]   |  

Abstract

Bone regeneration is an important issue in many situations, such as bone fracture and surgery. Umbilical cord mesenchymal stem cells (UC-MSCs) are promising cell sources for bone regeneration. Bone morphogenetic proteins and their bioactive peptides are biomolecules known to enhance the osteogenic differentiation of MSCs. However, fibrosis can arise during the development of implantable biomaterials. Therefore, it is important to control cell organization by enhancing osteogenic proliferation and differentiation and inhibiting fibroblast proliferation. Thus, we focused on the screening of such osteogenic-enhancing peptides. In the present study, we developed new peptide array screening platforms to evaluate cell proliferation and alkaline phosphatase activity in osteoblasts, UC-MSCs and fibroblasts. The conditions for the screening platform were first defined using UC-MSCs and an osteogenic differentiation peptide known as W9. Next, in silico screening to define the candidate peptides was carried out to evaluate the homology of 19 bone morphogenetic proteins. Twenty-five candidate 9-mer peptides were selected for screening. Finally, the screening of osteogenic-enhancing (osteogenic cell-selective proliferation and osteogenic differentiation) short peptide was carried out using the peptide array method, and three osteogenic-enhancing peptides were identified, confirming the validity of this screening. View Full-Text
Keywords: osteogenic peptides; peptide array; umbilical cord mesenchymal stem cells; bone morphogenetic proteins; bone regeneration osteogenic peptides; peptide array; umbilical cord mesenchymal stem cells; bone morphogenetic proteins; bone regeneration
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kanie, K.; Kurimoto, R.; Tian, J.; Ebisawa, K.; Narita, Y.; Honda, H.; Kato, R. Screening of Osteogenic-Enhancing Short Peptides from BMPs for Biomimetic Material Applications. Materials 2016, 9, 730.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top