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Materials 2016, 9(7), 543; doi:10.3390/ma9070543

Electrochemical Study and Characterization of an Amperometric Biosensor Based on the Immobilization of Laccase in a Nanostructure of TiO2 Synthesized by the Sol-Gel Method

1
Programa Institucional de Doctorado en Ciencias Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Santiago Tapia 403, col. Centro, Morelia, Mich. cp 58000, Mexico
2
Facultad de Ingeniería Civil, Universidad Michoacana de San Nicolás de Hidalgo, Santiago Tapia 403, col. Centro, Morelia, Mich cp 58000, Mexico
3
Facultad de Químico Farmacobiología, Universidad Michoacana de San Nicolás de Hidalgo, Santiago Tapia 403, col. Centro, Morelia, Mich cp 58000, Mexico
*
Author to whom correspondence should be addressed.
Academic Editors: Dusan Losic and Haolin Tang
Received: 4 May 2016 / Revised: 10 June 2016 / Accepted: 30 June 2016 / Published: 7 July 2016
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Abstract

Laccase amperometric biosensors were developed to detect the catechol compound. The laccase enzyme (LAC) immobilization was performed on nanostructures of (a) titania (TiO2); (b) titania/Nafion (TiO2/NAF) (both immobilized by the sol-gel method) and a third nanostructure, which consisted of a single biosensor composite of Nafion and laccase enzyme denoted as NAF/LAC. The Nafion was deposited on a graphite electrode and used to avoid “cracking” on the matrix. The TiO2 particle size was an average of 66 nm. FTIR spectroscopy vibration modes of different composites were determined. The electrochemical behavior of the biosensor was studied using electrochemical spectroscopy (EIS) and cyclic voltammetry (CV). The biosensor based on TiO2/NAF/LAC presented the best electro-chemical properties with regard to sensitivity, stability and detection limit after a period of 22 days. View Full-Text
Keywords: sol-gel; amperometric biosensors; sensitivity; stability; detection limit sol-gel; amperometric biosensors; sensitivity; stability; detection limit
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Romero-Arcos, M.; Garnica-Romo, M.G.; Martínez-Flores, H.E. Electrochemical Study and Characterization of an Amperometric Biosensor Based on the Immobilization of Laccase in a Nanostructure of TiO2 Synthesized by the Sol-Gel Method. Materials 2016, 9, 543.

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