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Materials 2016, 9(7), 532; doi:10.3390/ma9070532

Biomimetic Multispiked Connecting Ti-Alloy Scaffold Prototype for Entirely-Cementless Resurfacing Arthroplasty Endoprostheses—Exemplary Results of Implantation of the Ca-P Surface-Modified Scaffold Prototypes in Animal Model and Osteoblast Culture Evaluation

1
Department of Medical Bioengineering Fundamentals, Institute of Technology, Casimir the Great University, Karola Chodkiewicza Street 30, Bydgoszcz 85-064, Poland
2
Department of Process Engineering, Institute of Technology and Chemical Engineering, Poznan University of Technology, Marii Sklodowskiej-Curie 2, Poznan 60-965, Poland
3
Department of Spine Surgery, Oncologic Orthopaedics and Traumatology, Poznan University of Medical Sciences, 28 Czerwca 1956 135/147, Poznan 61-545, Poland
4
Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5A, Poznan 60-806, Poland
5
Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, Poznan 60-806, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Nicola Pugno
Received: 31 January 2016 / Revised: 7 June 2016 / Accepted: 23 June 2016 / Published: 29 June 2016
(This article belongs to the Special Issue Metallic Scaffolds for Bone Regeneration)
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Abstract

We present here—designed, manufactured, and tested by our research team—the Ti-alloy prototype of the multispiked connecting scaffold (MSC-Scaffold) interfacing the components of resurfacing arthroplasty (RA) endoprostheses with bone. The spikes of the MSC-Scaffold prototype mimic the interdigitations of the articular subchondral bone, which is the natural biostructure interfacing the articular cartilage with the periarticular trabecular bone. To enhance the osteoinduction/osteointegration potential of the MSC-Scaffold, the attempts to modify its bone contacting surfaces by the process of electrochemical cathodic deposition of Ca-P was performed with further immersion of the MSC-Scaffold prototypes in SBF in order to transform the amorphous calcium-phosphate coating in hydroxyapatite-like (HA-like) coating. The pilot experimental study of biointegration of unmodified and Ca-P surface-modified MSC-Scaffold prototypes was conducted in an animal model (swine) and in osteoblast cell culture. On the basis of a microscope-histological method the biointegration was proven by the presence of trabeculae in the interspike spaces of the MSC-Scaffold prototype on longitudinal and cross-sections of bone-implant specimens. The percentage of trabeculae in the area between the spikes of specimen containing Ca-P surface modified scaffold prototype observed in microCT reconstructions of the explanted joints was visibly higher than in the case of unmodified MSC-Scaffold prototypes. Significantly higher Alkaline Phosphatase (ALP) activity and the cellular proliferation in the case of Ca-P-modified MSC-Scaffold pre-prototypes, in comparison with unmodified pre-prototypes, was found in osteoblast cell cultures. The obtained results of experimental implantation in an animal model and osteoblast cell culture evaluations of Ca-P surface-modified and non-modified biomimetic MSC-Scaffold prototypes for biomimetic entirely-cementless RA endoprostheses indicate the enhancement of the osteoinduction/osteointegration potential by the Ca-P surface modification of the Ti-alloy MSC-Scaffold prototype. Planned further research on the prototype of this biomimetic MSC-Scaffold for a new generation of RA endoprostheses is also given. View Full-Text
Keywords: multispiked connecting scaffold (MSC-Scaffold) Ti-alloy prototype; bone-implant biomimetic prototype interface; osteoinduction and osteointegration potential; resurfacing arthroplasty RA endoprostheses; animal model evaluation; osteoblast cell culture evaluation multispiked connecting scaffold (MSC-Scaffold) Ti-alloy prototype; bone-implant biomimetic prototype interface; osteoinduction and osteointegration potential; resurfacing arthroplasty RA endoprostheses; animal model evaluation; osteoblast cell culture evaluation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Uklejewski, R.; Rogala, P.; Winiecki, M.; Tokłowicz, R.; Ruszkowski, P.; Wołuń-Cholewa, M. Biomimetic Multispiked Connecting Ti-Alloy Scaffold Prototype for Entirely-Cementless Resurfacing Arthroplasty Endoprostheses—Exemplary Results of Implantation of the Ca-P Surface-Modified Scaffold Prototypes in Animal Model and Osteoblast Culture Evaluation. Materials 2016, 9, 532.

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