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Materials 2016, 9(5), 381; doi:10.3390/ma9050381

Higher Ratios of Hyaluronic Acid Enhance Chondrogenic Differentiation of Human MSCs in a Hyaluronic Acid–Gelatin Composite Scaffold

1
Department of Trauma Surgery, University Medical CentreRegensburg, Franz-Josef-Strauss-Allee 11, Regensburg 93053, Germany
2
Sporthopaedicum, Straubing/Regensburg, Hildegard-von-Bingen-Str. 1, Regensburg 93053, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Nicola Pugno
Received: 10 March 2016 / Revised: 26 April 2016 / Accepted: 10 May 2016 / Published: 17 May 2016
(This article belongs to the Special Issue Regenerative Materials)
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Abstract

Mesenchymal stem cells (MSCs) seeded on specific carrier materials are a promising source for the repair of traumatic cartilage injuries. The best supportive carrier material has not yet been determined. As natural components of cartilage’s extracellular matrix, hyaluronic acid and collagen are the focus of biomaterial research. In order to optimize chondrogenic support, we investigated three different scaffold compositions of a hyaluronic acid (HA)-gelatin based biomaterial. Methods: Human MSCs (hMSCs) were seeded under vacuum on composite scaffolds of three different HA-gelatin ratios and cultured in chondrogenic medium for 21 days. Cell-scaffold constructs were assessed at different time points for cell viability, gene expression patterns, production of cartilage-specific extracellular matrix (ECM) and for (immuno-)histological appearance. The intrinsic transforming growth factor beta (TGF-beta) uptake of empty scaffolds was evaluated by determination of the TGF-beta concentrations in the medium over time. Results: No significant differences were found for cell seeding densities and cell viability. hMSCs seeded on scaffolds with higher ratios of HA showed better cartilage-like differentiation in all evaluated parameters. TGF-beta uptake did not differ between empty scaffolds. Conclusion: Higher ratios of HA support the chondrogenic differentiation of hMSCs seeded on a HA-gelatin composite scaffold. View Full-Text
Keywords: composite scaffold; hyaluronic acid; gelatin; chondrogenesis; human mesenchymal stem cells composite scaffold; hyaluronic acid; gelatin; chondrogenesis; human mesenchymal stem cells
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MDPI and ACS Style

Pfeifer, C.G.; Berner, A.; Koch, M.; Krutsch, W.; Kujat, R.; Angele, P.; Nerlich, M.; Zellner, J. Higher Ratios of Hyaluronic Acid Enhance Chondrogenic Differentiation of Human MSCs in a Hyaluronic Acid–Gelatin Composite Scaffold. Materials 2016, 9, 381.

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