Next Article in Journal
Fabrication of Bendable Circuits on a Polydimethylsiloxane (PDMS) Surface by Inkjet Printing Semi-Wrapped Structures
Next Article in Special Issue
Screening of Osteogenic-Enhancing Short Peptides from BMPs for Biomimetic Material Applications
Previous Article in Journal
Effective Crack Control of Concrete by Self-Healing of Cementitious Composites Using Synthetic Fiber
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessFeature PaperArticle
Materials 2016, 9(4), 249; doi:10.3390/ma9040249

Rapid Assay to Assess Bacterial Adhesion on Textiles

1
Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland
2
Laboratory for Advanced Fibers, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland
3
Sanitized AG, Burgdorf 3400, Switzerland
4
Swissatest Testmaterials AG, St. Gallen 9015, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Jaroslaw W. Drelich
Received: 22 February 2016 / Revised: 16 March 2016 / Accepted: 22 March 2016 / Published: 30 March 2016
(This article belongs to the Special Issue Advances in Biointerfaces)
View Full-Text   |   Download PDF [2486 KB, uploaded 30 March 2016]   |  

Abstract

Textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. Biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. To develop novel anti-adhesive textile coatings, a rapid, reliable, and quantitative high-throughput method to study microbial attachment to fabrics is required, however currently not available. Here, a fast and reliable 96-well plate-based screening method is developed. The quantification of bacterial adhesion is based on nucleic acid staining by SYTO9, with Pseudomonas aeruginosa and Staphylococcus aureus as the model microorganisms. Subsequently, 38 commercially available and novel coatings were evaluated for their anti-bacterial adhesion properties. A poly(l-lysine)-g-poly(ethylene glycol) coating on polyester textile substratum revealed an 80% reduction of bacterial adhesion. Both the coating itself and the anti-adhesive property were stable after 20 washing cycles, confirmed by X-ray analysis. The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment. View Full-Text
Keywords: antifouling; bacterial adhesion; microtiter plate; biofilm; textile coating antifouling; bacterial adhesion; microtiter plate; biofilm; textile coating
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Schmidt-Emrich, S.; Stiefel, P.; Rupper, P.; Katzenmeier, H.; Amberg, C.; Maniura-Weber, K.; Ren, Q. Rapid Assay to Assess Bacterial Adhesion on Textiles. Materials 2016, 9, 249.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top