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Materials 2016, 9(4), 249; doi:10.3390/ma9040249

Rapid Assay to Assess Bacterial Adhesion on Textiles

Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland
Laboratory for Advanced Fibers, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen 9014, Switzerland
Sanitized AG, Burgdorf 3400, Switzerland
Swissatest Testmaterials AG, St. Gallen 9015, Switzerland
Author to whom correspondence should be addressed.
Academic Editor: Jaroslaw W. Drelich
Received: 22 February 2016 / Revised: 16 March 2016 / Accepted: 22 March 2016 / Published: 30 March 2016
(This article belongs to the Special Issue Advances in Biointerfaces)
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Textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. Biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. To develop novel anti-adhesive textile coatings, a rapid, reliable, and quantitative high-throughput method to study microbial attachment to fabrics is required, however currently not available. Here, a fast and reliable 96-well plate-based screening method is developed. The quantification of bacterial adhesion is based on nucleic acid staining by SYTO9, with Pseudomonas aeruginosa and Staphylococcus aureus as the model microorganisms. Subsequently, 38 commercially available and novel coatings were evaluated for their anti-bacterial adhesion properties. A poly(l-lysine)-g-poly(ethylene glycol) coating on polyester textile substratum revealed an 80% reduction of bacterial adhesion. Both the coating itself and the anti-adhesive property were stable after 20 washing cycles, confirmed by X-ray analysis. The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment. View Full-Text
Keywords: antifouling; bacterial adhesion; microtiter plate; biofilm; textile coating antifouling; bacterial adhesion; microtiter plate; biofilm; textile coating

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Schmidt-Emrich, S.; Stiefel, P.; Rupper, P.; Katzenmeier, H.; Amberg, C.; Maniura-Weber, K.; Ren, Q. Rapid Assay to Assess Bacterial Adhesion on Textiles. Materials 2016, 9, 249.

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