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Materials 2016, 9(11), 889; doi:10.3390/ma9110889

A Natural Bacterium-Produced Membrane-Bound Nanocarrier for Drug Combination Therapy

1
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
2
Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China
3
Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jung Ho Je
Received: 17 September 2016 / Revised: 30 October 2016 / Accepted: 31 October 2016 / Published: 2 November 2016
(This article belongs to the Section Structure Analysis and Characterization)
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Abstract

To minimize the non-specific toxicity of drug combination during cancer therapy, we prepared a new system synthesized from bacteria to deliver the anticancer drugs cytosine arabinoside (Ara-C) and daunorubicin (DNR). In this study, we selected genipin (GP) and poly-l-glutamic acid (PLGA) as dual crosslinkers. Herewith, we demonstrated the preparation, characterization and in vitro antitumor effects of Ara-C and DNR loaded GP-PLGA-modified bacterial magnetosomes (BMs) (ADBMs-P). The results show that this new system is stable and exhibits optimal drug-loading properties. The average diameters of BMs and ADBMs-P were 42.0 ± 8.6 nm and 65.5 ± 8.9 nm, respectively, and the zeta potential of ADBMs-P (−42.0 ± 6.4 mV) was significantly less than that of BMs (−28.6 ± 7.6 mV). The optimal encapsulation efficiency and drug loading of Ara-C were 68.4% ± 9.4% and 32.4% ± 2.9%, respectively, and those of DNR were 36.1% ± 2.5% and 17.9% ± 1.6%. Interestingly, this system also exhibits long-term release behaviour sequentially, without an initial burst release. The Ara-C drug continued to release about 85% within 40 days, while DNR release lasted only for 13 days. Moreover, similar to free drugs, ADBMs-Ps are strongly cytotoxic to cancer cells in vitro (HL-60 cells), with the inhibition rate approximately 96%. This study reveals that this new system has a potential for drug delivery application in the future, especially for combination therapy. View Full-Text
Keywords: magnetosomes; natural carrier; drug combination therapy; dual crosslinkers magnetosomes; natural carrier; drug combination therapy; dual crosslinkers
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MDPI and ACS Style

Long, R.; Liu, Y.; Dai, Q.; Wang, S.; Deng, Q.; Zhou, X. A Natural Bacterium-Produced Membrane-Bound Nanocarrier for Drug Combination Therapy. Materials 2016, 9, 889.

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