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Materials 2016, 9(10), 857; doi:10.3390/ma9100857

Gold Nanoparticle-Based Colorimetric and Electrochemical Methods for Dipeptidyl Peptidase-IV Activity Assay and Inhibitor Screening

Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, China
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Academic Editor: Ilaria Fratoddi
Received: 3 September 2016 / Revised: 30 September 2016 / Accepted: 13 October 2016 / Published: 21 October 2016
(This article belongs to the Special Issue Noble Metal Nanoparticles)
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Abstract

We presented the colorimetric and electrochemical methods for determination of the dipeptidyl peptidase-IV (DPP-IV) activity and screening of its inhibitor using gold nanoparticle (AuNP) as the probe. In the colorimetric assay, the substrate peptide with a sequence of Arg-Pro-Arg induced the aggregation and color change of AuNPs, whereas cleavage of the peptide by DPP-IV prevented the aggregation of AuNPs. Furthermore, the aggregation of AuNPs in the solution was easily initiated on a solid/liquid (electrode/electrolyte) surface, which induced a decrease in the electron-transfer resistance. However, once the peptide was clipped by DPP-IV, the assembly of AuNPs on electrode surface was prevented. Consequently, a higher electron-transfer resistance was observed. The colorimetric and electrochemical assays allowed for the determination of DPP-IV with the detection limits of 70 μU/mL and 0.55 μU/mL, respectively. Meanwhile, the proposed methods were used to determine DPP-IV inhibitor with satisfactory results. Both the colorimetric and electrochemical methods are simple, rapid and sufficiently sensitive for DPP-IV activity assay and inhibitor screening. The results also demonstrated that the AuNP-based colorimetric assay could be converted into an enhanced surface tethered electrochemical assay with improving sensitivity. The simple detection principle may be extended to the design of other peptidases biosensors with easy manipulation procedures. View Full-Text
Keywords: dipeptidyl peptidase-IV; gold nanoparticles; colorimetric assay; electrochemical impedance spectroscopy; inhibitor screening dipeptidyl peptidase-IV; gold nanoparticles; colorimetric assay; electrochemical impedance spectroscopy; inhibitor screening
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Xia, N.; Wang, X.; Wang, X.; Zhou, B. Gold Nanoparticle-Based Colorimetric and Electrochemical Methods for Dipeptidyl Peptidase-IV Activity Assay and Inhibitor Screening. Materials 2016, 9, 857.

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