Antimicrobial Properties and Cytocompatibility of PLGA/Ag Nanocomposites
AbstractThe purpose of this study was to investigate the antimicrobial properties of multifunctional nanocomposites based on poly(dl-Lactide-co-Glycolide) (PLGA) and increasing concentration of silver (Ag) nanoparticles and their effects on cell viability for biomedical applications. PLGA nanocomposite films, produced by solvent casting with 1 wt%, 3 wt% and 7 wt% of Ag nanoparticles were investigated and surface properties were characterized by atomic force microscopy and contact angle measurements. Antibacterial tests were performed using an Escherichia coli RB and Staphylococcus aureus 8325-4 strains. The cell viability and morphology were performed with a murine fibroblast cell line (L929) and a human osteosarcoma cell line (SAOS-2) by cell viability assay and electron microscopy observations. Matrix protein secretion and deposition were also quantified by enzyme-linked immunosorbent assay (ELISA). The results suggest that the PLGA film morphology can be modified introducing a small percentage of silver nanoparticles, which induce the onset of porous round-like microstructures and also affect the wettability. The PLGA/Ag films having silver nanoparticles of more than 3 wt% showed antibacterial effects against E. coli and S. aureus. Furthermore, silver-containing PLGA films displayed also a good cytocompatibility when assayed with L929 and SAOS-2 cells; indicating the PLGA/3Ag nanocomposite film as a promising candidate for tissue engineering applications. View Full-Text
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Scavone, M.; Armentano, I.; Fortunati, E.; Cristofaro, F.; Mattioli, S.; Torre, L.; Kenny, J.M.; Imbriani, M.; Arciola, C.R.; Visai, L. Antimicrobial Properties and Cytocompatibility of PLGA/Ag Nanocomposites. Materials 2016, 9, 37.
Scavone M, Armentano I, Fortunati E, Cristofaro F, Mattioli S, Torre L, Kenny JM, Imbriani M, Arciola CR, Visai L. Antimicrobial Properties and Cytocompatibility of PLGA/Ag Nanocomposites. Materials. 2016; 9(1):37.Chicago/Turabian Style
Scavone, Mariangela; Armentano, Ilaria; Fortunati, Elena; Cristofaro, Francesco; Mattioli, Samantha; Torre, Luigi; Kenny, Jose M.; Imbriani, Marcello; Arciola, Carla R.; Visai, Livia. 2016. "Antimicrobial Properties and Cytocompatibility of PLGA/Ag Nanocomposites." Materials 9, no. 1: 37.
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