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Materials 2014, 7(8), 5554-5564; doi:10.3390/ma7085554
Article

Synthesis of Water-Dispersed Ferrecene/Phenylboronic Acid-Modified Bifunctional Gold Nanoparticles and the Application in Biosensing

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College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, China
* Author to whom correspondence should be addressed.
Received: 9 May 2014 / Revised: 17 July 2014 / Accepted: 24 July 2014 / Published: 31 July 2014
(This article belongs to the Special Issue Advanced Nanomaterials for Biosensors)
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Abstract

Phenylboronic acids can form tight covalent bonds with diol-containing biomolecules. In this work, water-dispersed bifunctional gold nanoparticles (AuNPs) modified with ferrecene (Fc)-derivatized peptides and 4-mercaptophenylboronic acids (MBA) (denoted as Fc–MBA–AuNPs) were synthesized and characterized by UV/vis spectrometry and transmission electron microscopy. To demonstrate the application and the analytical merits of the nanoparticles in biosensing, glycoprotein avidin was tested as a model analyte. Specifically, avidin was captured by the biotin-covered gold electrode via the strong biotin-avidin interaction. Then, Fc–MBA–AuNPs were attached by the captured avidin through the formation of tight covalent bonds between the boronic acid moieties of Fc–MBA–AuNPs and the oligosaccharides of avidin. As a result, a detection limit of 0.2 pM was achieved. We believe that the bifunctional nanoparticles would found many applications in amplified detection of diol-containing species by rational design of the surface chemistry of electrode.
Keywords: gold nanoparticles; ferrecene; boronic acid; biosensors; diols gold nanoparticles; ferrecene; boronic acid; biosensors; diols
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Xing, Y.; Liu, L.; Zhao, D.; Yang, Y.; Chu, X. Synthesis of Water-Dispersed Ferrecene/Phenylboronic Acid-Modified Bifunctional Gold Nanoparticles and the Application in Biosensing. Materials 2014, 7, 5554-5564.

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