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Materials 2014, 7(3), 1555-1572; doi:10.3390/ma7031555
Article

Uptake of Eudragit Retard L (Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats

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Received: 18 October 2013; in revised form: 18 November 2013 / Accepted: 18 February 2014 / Published: 28 February 2014
(This article belongs to the Special Issue Nanocomposites of Polymers and Inorganic Particles 2013)
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Abstract: The aim of this study was to prepare Eudragit Retard L (Eudragit RL) nanoparticles (ENPs) and to determine their properties, their uptake by the human THP-1 cell line in vitro and their effect on the hematological parameters and erythrocyte damage in rats. ENPs showed an average size of 329.0 ± 18.5 nm, a positive zeta potential value of +57.5 ± 5.47 mV and nearly spherical shape with a smooth surface. THP-1 cell lines could phagocyte ENPs after 2 h of incubation. In the in vivo study, male Sprague-Dawley rats were exposed orally or intraperitoneally (IP) with a single dose of ENP (50 mg/kg body weight). Blood samples were collected after 4 h, 48 h, one week and three weeks for hematological and erythrocytes analysis. ENPs induced significant hematological disturbances in platelets, red blood cell (RBC) total and differential counts of white blood cells (WBCs) after 4 h, 48 h and one week. ENP increased met-Hb and Co-Hb derivatives and decreased met-Hb reductase activity. These parameters were comparable to the control after three weeks when administrated orally. It could be concluded that the route of administration has a major effect on the induction of hematological disturbances and should be considered when ENPs are applied for drug delivery systems.
Keywords: Eudragit; nanoparticles; nanotoxicity; hematology; erythrocytes damage; oxidative stress Eudragit; nanoparticles; nanotoxicity; hematology; erythrocytes damage; oxidative stress
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Abdel-Wahhab, M.A.; Abdel-Wahhab, K.G.; Mannaa, F.A.; Hassan, N.S.; Safar, R.; Diab, R.; Foliguet, B.; Ferrari, L.; Rihn, B.H. Uptake of Eudragit Retard L (Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats. Materials 2014, 7, 1555-1572.

AMA Style

Abdel-Wahhab MA, Abdel-Wahhab KG, Mannaa FA, Hassan NS, Safar R, Diab R, Foliguet B, Ferrari L, Rihn BH. Uptake of Eudragit Retard L (Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats. Materials. 2014; 7(3):1555-1572.

Chicago/Turabian Style

Abdel-Wahhab, Mosaad A.; Abdel-Wahhab, Khaled G.; Mannaa, Fathia A.; Hassan, Nabila S.; Safar, Ramia; Diab, Roudayna; Foliguet, Bernard; Ferrari, Luc; Rihn, Bertrand H. 2014. "Uptake of Eudragit Retard L (Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats." Materials 7, no. 3: 1555-1572.


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