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Materials 2013, 6(7), 2978-2988; doi:10.3390/ma6072978
Article

In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel

1,#
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2,#
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1
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2,3
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2,3,*  and 1,*
1 Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea 2 Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110-810, Korea 3 Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul 110-744, Korea # These authors contribute equally to the manuscript.
* Authors to whom correspondence should be addressed.
Received: 14 June 2013 / Revised: 8 July 2013 / Accepted: 11 July 2013 / Published: 17 July 2013
(This article belongs to the Section Biomaterials)
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Abstract

In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and osteogenic factors was injected into nude mice and developed into in situ-forming hydrogels at the injection sites; these hydrogels maintained their shape even after 12 weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed only in the in situ gel-forming MCL hydrogel in the presence of hEBs and osteogenic factors. In conclusion, this preliminary study suggests that hEBs and osteogenic factors embedded in an in situ-forming MCL hydrogel may provide numerous benefits as a noninvasive alternative for allogeneic tissue engineering applications.
Keywords: human embryoid bodies; in situ-forming hydrogel; osteogenic differentiation; tissue engineering human embryoid bodies; in situ-forming hydrogel; osteogenic differentiation; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kim, D.Y.; Kim, Y.Y.; Lee, H.B.; Moon, S.Y.; Ku, S.-Y.; Kim, M.S. In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel. Materials 2013, 6, 2978-2988.

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