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In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel
Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea
Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110-810, Korea
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul 110-744, Korea
These authors contribute equally to the manuscript.
* Authors to whom correspondence should be addressed.
Received: 14 June 2013; in revised form: 8 July 2013 / Accepted: 11 July 2013 / Published: 17 July 2013
Abstract: In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and osteogenic factors was injected into nude mice and developed into in situ-forming hydrogels at the injection sites; these hydrogels maintained their shape even after 12 weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed only in the in situ gel-forming MCL hydrogel in the presence of hEBs and osteogenic factors. In conclusion, this preliminary study suggests that hEBs and osteogenic factors embedded in an in situ-forming MCL hydrogel may provide numerous benefits as a noninvasive alternative for allogeneic tissue engineering applications.
Keywords: human embryoid bodies; in situ-forming hydrogel; osteogenic differentiation; tissue engineering
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MDPI and ACS Style
Kim, D.Y.; Kim, Y.Y.; Lee, H.B.; Moon, S.Y.; Ku, S.-Y.; Kim, M.S. In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel. Materials 2013, 6, 2978-2988.
Kim DY, Kim YY, Lee HB, Moon SY, Ku S-Y, Kim MS. In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel. Materials. 2013; 6(7):2978-2988.
Kim, Da Y.; Kim, Yoon Y.; Lee, Hai B.; Moon, Shin Y.; Ku, Seung-Yup; Kim, Moon S. 2013. "In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel." Materials 6, no. 7: 2978-2988.