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Materials 2013, 6(7), 2978-2988; doi:10.3390/ma6072978

In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel

1
Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea
2
Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul 110-810, Korea
3
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul 110-744, Korea
#
These authors contribute equally to the manuscript.
*
Authors to whom correspondence should be addressed.
Received: 14 June 2013 / Revised: 8 July 2013 / Accepted: 11 July 2013 / Published: 17 July 2013
(This article belongs to the Section Biomaterials)
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Abstract

In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and osteogenic factors was injected into nude mice and developed into in situ-forming hydrogels at the injection sites; these hydrogels maintained their shape even after 12 weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed only in the in situ gel-forming MCL hydrogel in the presence of hEBs and osteogenic factors. In conclusion, this preliminary study suggests that hEBs and osteogenic factors embedded in an in situ-forming MCL hydrogel may provide numerous benefits as a noninvasive alternative for allogeneic tissue engineering applications.
Keywords: human embryoid bodies; in situ-forming hydrogel; osteogenic differentiation; tissue engineering human embryoid bodies; in situ-forming hydrogel; osteogenic differentiation; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kim, D.Y.; Kim, Y.Y.; Lee, H.B.; Moon, S.Y.; Ku, S.-Y.; Kim, M.S. In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel. Materials 2013, 6, 2978-2988.

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