Abstract: This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments (0/90° lay-down pattern), with a diameter of around 365 μm and interfilament distance of around 191 μm, were produced using a melt extrusion-based additive manufacturing technique. PLGA electrospun fibers with a diameter of around 1 μm were collected on top of the PCL constructs with different thicknesses, showing a certain degree of alignment. Cell culture experiments employing the MC3T3 murine preosteoblast cell line showed good cell viability and adhesion on the dual-scale scaffolds. In particular, the influence of electrospun fibers on cell morphology and behavior was evident, as well as in creating a structural bridging for cell colonization in the interfilament gap.
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Mota, C.; Puppi, D.; Dinucci, D.; Errico, C.; Bártolo, P.; Chiellini, F. Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering. Materials 2011, 4, 527-542.
Mota C, Puppi D, Dinucci D, Errico C, Bártolo P, Chiellini F. Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering. Materials. 2011; 4(3):527-542.
Mota, Carlos; Puppi, Dario; Dinucci, Dinuccio; Errico, Cesare; Bártolo, Paulo; Chiellini, Federica. 2011. "Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering." Materials 4, no. 3: 527-542.