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A New Highly Bioactive Composite for Scaffold Applications: A Feasibility Study
Materials 2011, 4(3), 527-542; doi:10.3390/ma4030527
Article

Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering

1
,
1
,
1
,
1
,
2
 and
1,*
1 Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab), Department of Chemistry and Industrial Chemistry, University of Pisa, via Vecchia Livornese 1291, 56010 San Piero a Grado (Pi), Italy 2 Centre for Rapid and Sustainable Product Development, Centro Empresarial da Marinha Grande, Rua de Portugal—Zona Industrial, 2430-028 Marinha Grande, Portugal
* Author to whom correspondence should be addressed.
Received: 17 January 2011 / Accepted: 25 February 2011 / Published: 1 March 2011
(This article belongs to the Special Issue Tissue Engineering Scaffolds)
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Abstract

This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments (0/90° lay-down pattern), with a diameter of around 365 μm and interfilament distance of around 191 μm, were produced using a melt extrusion-based additive manufacturing technique. PLGA electrospun fibers with a diameter of around 1 μm were collected on top of the PCL constructs with different thicknesses, showing a certain degree of alignment. Cell culture experiments employing the MC3T3 murine preosteoblast cell line showed good cell viability and adhesion on the dual-scale scaffolds. In particular, the influence of electrospun fibers on cell morphology and behavior was evident, as well as in creating a structural bridging for cell colonization in the interfilament gap.
Keywords: additive manufacturing; electrospinning; scaffold; tissue engineering additive manufacturing; electrospinning; scaffold; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Mota, C.; Puppi, D.; Dinucci, D.; Errico, C.; Bártolo, P.; Chiellini, F. Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering. Materials 2011, 4, 527-542.

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