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Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering
Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab), Department of Chemistry and Industrial Chemistry, University of Pisa, via Vecchia Livornese 1291, 56010 San Piero a Grado (Pi), Italy
Centre for Rapid and Sustainable Product Development, Centro Empresarial da Marinha Grande, Rua de Portugal—Zona Industrial, 2430-028 Marinha Grande, Portugal
* Author to whom correspondence should be addressed.
Received: 17 January 2011; Accepted: 25 February 2011 / Published: 1 March 2011
Abstract: This research activity was aimed at the development of dual-scale scaffolds consisting of three-dimensional constructs of aligned poly(ε-caprolactone) (PCL) microfilaments and electrospun poly(lactic-co-glycolic acid) (PLGA) fibers. PCL constructs composed by layers of parallel microsized filaments (0/90° lay-down pattern), with a diameter of around 365 μm and interfilament distance of around 191 μm, were produced using a melt extrusion-based additive manufacturing technique. PLGA electrospun fibers with a diameter of around 1 μm were collected on top of the PCL constructs with different thicknesses, showing a certain degree of alignment. Cell culture experiments employing the MC3T3 murine preosteoblast cell line showed good cell viability and adhesion on the dual-scale scaffolds. In particular, the influence of electrospun fibers on cell morphology and behavior was evident, as well as in creating a structural bridging for cell colonization in the interfilament gap.
Keywords: additive manufacturing; electrospinning; scaffold; tissue engineering
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Mota, C.; Puppi, D.; Dinucci, D.; Errico, C.; Bártolo, P.; Chiellini, F. Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering. Materials 2011, 4, 527-542.
Mota C, Puppi D, Dinucci D, Errico C, Bártolo P, Chiellini F. Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering. Materials. 2011; 4(3):527-542.
Mota, Carlos; Puppi, Dario; Dinucci, Dinuccio; Errico, Cesare; Bártolo, Paulo; Chiellini, Federica. 2011. "Dual-Scale Polymeric Constructs as Scaffolds for Tissue Engineering." Materials 4, no. 3: 527-542.