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Tissue Equivalents Based on Cell-Seeded Biodegradable Microfluidic Constructs
Biomedical Engineering Center, Draper Laboratory, 555 Technology Square, Cambridge MA, USA
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford MA, USA
Center for Innovations in Wound Healing Research, Tufts University School of Medicine, 150 Harrison Avenue, Boston MA, USA
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 28 January 2010; in revised form: 6 March 2010 / Accepted: 11 March 2010 / Published: 15 March 2010
Abstract: One of the principal challenges in the field of tissue engineering and regenerative medicine is the formation of functional microvascular networks capable of sustaining tissue constructs. Complex tissues and vital organs require a means to support oxygen and nutrient transport during the development of constructs both prior to and after host integration, and current approaches have not demonstrated robust solutions to this challenge. Here, we present a technology platform encompassing the design, construction, cell seeding and functional evaluation of tissue equivalents for wound healing and other clinical applications. These tissue equivalents are comprised of biodegradable microfluidic scaffolds lined with microvascular cells and designed to replicate microenvironmental cues necessary to generate and sustain cell populations to replace dermal and/or epidermal tissues lost due to trauma or disease. Initial results demonstrate that these biodegradable microfluidic devices promote cell adherence and support basic cell functions. These systems represent a promising pathway towards highly integrated three-dimensional engineered tissue constructs for a wide range of clinical applications.
Keywords: microfluidics; microfabrication; tissue engineering; wound healing; vasculature
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Borenstein, J.T.; Megley, K.; Wall, K.; Pritchard, E.M.; Truong, D.; Kaplan, D.L.; Tao, S.L.; Herman, I.M. Tissue Equivalents Based on Cell-Seeded Biodegradable Microfluidic Constructs. Materials 2010, 3, 1833-1844.
Borenstein JT, Megley K, Wall K, Pritchard EM, Truong D, Kaplan DL, Tao SL, Herman IM. Tissue Equivalents Based on Cell-Seeded Biodegradable Microfluidic Constructs. Materials. 2010; 3(3):1833-1844.
Borenstein, Jeffrey T.; Megley, Katie; Wall, Kimberly; Pritchard, Eleanor M.; Truong, David; Kaplan, David L.; Tao, Sarah L.; Herman, Ira M. 2010. "Tissue Equivalents Based on Cell-Seeded Biodegradable Microfluidic Constructs." Materials 3, no. 3: 1833-1844.