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Materials 2010, 3(11), 4994-5006; doi:10.3390/ma3114994
Article

Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry

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Received: 25 October 2010 / Accepted: 9 November 2010 / Published: 22 November 2010
(This article belongs to the Special Issue Supported Lipid Membrane Surface Modifications and Applications)
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Abstract

We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cyclo[RGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence. Biotinylated AK-cyclo[RGDfC] is bound to a supported lipid bilayer via a streptavidin interlayer. Layering, hydration and packing of the coating is quantified by X-ray and neutron reflectometry experiments. AK-cyclo[RGDfC] binds to the streptavidin interlayer in a stretched-out on edge configuration. The highly packed configuration with only 12% water content maximizes the number of accessible adhesion sites. Enhanced cell spreading of neural stem cells was observed for AK-cyclo[RGDfC] functionalized bilayers. Due to the large variety of surfaces which can be coated by physisorption of lipid bilayers, this approach is of general interest for the fabrication of biocompatible surfaces.
Keywords: supported lipid bilayers; biomimetics; bionanotechnology; functional coatings; self-assembly supported lipid bilayers; biomimetics; bionanotechnology; functional coatings; self-assembly
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huth, M.; Hertrich, S.; Mezo, G.; Madarasz, E.; Nickel, B. Neural Stem Cell Spreading on Lipid Based Artificial Cell Surfaces, Characterized by Combined X-ray and Neutron Reflectometry. Materials 2010, 3, 4994-5006.

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