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Materials 2010, 3(10), 4784-4792; doi:10.3390/ma3104784

Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis

Proteomic Laboratory, iRCM, CEA, Fontenay aux Roses, France
Received: 8 October 2010 / Revised: 13 October 2010 / Accepted: 15 October 2010 / Published: 20 October 2010
(This article belongs to the Special Issue Advances in Dyes and Pigments)
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Abstract

Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was investigated. Visible dyes (Coomassie blue and silver nitrate), fluorescent dyes (Sypro Ruby, Deep Purple) and cyanine labeled methods were compared.
Keywords: protein staining; fluorescent dyes; two-dimensional electrophoresis; proteomics protein staining; fluorescent dyes; two-dimensional electrophoresis; proteomics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chevalier, F. Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis. Materials 2010, 3, 4784-4792.

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