Materials 2010, 3(10), 4784-4792; doi:10.3390/ma3104784
Review

Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis

email
Received: 8 October 2010; in revised form: 13 October 2010 / Accepted: 15 October 2010 / Published: 20 October 2010
(This article belongs to the Special Issue Advances in Dyes and Pigments)
Download PDF [299 KB, uploaded 20 October 2010]
Abstract: Staining of two-dimensional gels is a primary concern in proteomic studies using two-dimensional gel electrophoresis with respect to the number of proteins analyzed, the accuracy of spot quantification and reproducibility. In this review article, the efficiency of the most widely used dyes was investigated. Visible dyes (Coomassie blue and silver nitrate), fluorescent dyes (Sypro Ruby, Deep Purple) and cyanine labeled methods were compared.
Keywords: protein staining; fluorescent dyes; two-dimensional electrophoresis; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Chevalier, F. Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis. Materials 2010, 3, 4784-4792.

AMA Style

Chevalier F. Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis. Materials. 2010; 3(10):4784-4792.

Chicago/Turabian Style

Chevalier, François. 2010. "Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis." Materials 3, no. 10: 4784-4792.


Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert