Electrospun Bilayer Chitosan/Hyaluronan Material and Its Compatibility with Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Characterization of Polysaccharides
2.2. Polymer Solutions
2.3. Electrospinning
2.4. Film Preparation
2.5. General Methods
2.6. Cell Culture and in Vitro Tests
3. Results
3.1. Preparation and SEM Morphology of CS and CS–HA Nonwoven Materials
3.2. Swelling
3.3. X-Ray Diffraction
3.4. Porosity
3.5. Mechanical Tests
3.6. Roughness
3.7. Biocompatibility Testing
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Entry | Sample | Treatment | Swelling in Water, g/g | Swelling in 0.9% NaCl, g/g |
---|---|---|---|---|
1 | CS-nonwoven | 80 °С, four hours | 5.3 ± 0.4 | 2.2 ± 0.2 |
2 | CS–HA-nonwoven | 80 °С, four hours | 1.4 ± 0.1 | 1.0 ± 0.1 |
3 | CS–HA-nonwoven | 100 °С, two hours | 2.8 ± 0.2 | 2.3 ± 0.2 |
Parameter | CS-Film [32] | CS-Nonwoven | CS–HA-Nonwoven |
---|---|---|---|
Average logarithmic pore radius, nm | 1.17 | 1.62 | 1.89 |
Average pore radius, nm | 241 | 489 | 416 |
Porosity over weight, cm3/g | 0.29 | 9.36 | 6.02 |
Porosity over volume, cm3/cm3 | 0.29 | 0.976 | 0.587 |
Meso- and macro-pore surface over weight, m2/g | 29.9 | 878 | 950 |
Meso- and macro-pore surface over volume, m2/cm3 | 29.2 | 89.1 | 92.7 |
Total pore surface over weight, m2/g | 23.3 | 2464 | 1027 |
Total pore surface over volume, m2/cm3 | 22.8 | 257 | 100 |
Sample | d, μm | E, MPa | σy, MPa | σb, MPa | εb, % |
---|---|---|---|---|---|
CS-nonwoven | 40–42 | 253 ± 23 | 9.7 ± 0.7 | 9.4 ± 0.8 | 6.9 ± 0.8 |
CS–HA-nonwoven | 30–31 | 555 ± 25 | - | 22.2 ± 0.9 | 6.5 ± 0.3 |
Sample | Adhered Cells, cells/mm2 | Living Cells, % | Late Apoptosis/Necrosis, % | Early Apoptosis, % |
---|---|---|---|---|
CS-nonwoven | 281 ± 18 | 84.1 ± 1.81 | 10.2 ± 1.21 | 4.7 ± 0.5 |
CS–НА-nonwoven | 397 ± 39 | 89.6 ± 0.81 | 5.3 ± 0.31 | 3.6 ± 0.5 |
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Petrova, V.A.; Chernyakov, D.D.; Poshina, D.N.; Gofman, I.V.; Romanov, D.P.; Mishanin, A.I.; Golovkin, A.S.; Skorik, Y.A. Electrospun Bilayer Chitosan/Hyaluronan Material and Its Compatibility with Mesenchymal Stem Cells. Materials 2019, 12, 2016. https://doi.org/10.3390/ma12122016
Petrova VA, Chernyakov DD, Poshina DN, Gofman IV, Romanov DP, Mishanin AI, Golovkin AS, Skorik YA. Electrospun Bilayer Chitosan/Hyaluronan Material and Its Compatibility with Mesenchymal Stem Cells. Materials. 2019; 12(12):2016. https://doi.org/10.3390/ma12122016
Chicago/Turabian StylePetrova, Valentina A., Daniil D. Chernyakov, Daria N. Poshina, Iosif V. Gofman, Dmitry P. Romanov, Alexander I. Mishanin, Alexey S. Golovkin, and Yury A. Skorik. 2019. "Electrospun Bilayer Chitosan/Hyaluronan Material and Its Compatibility with Mesenchymal Stem Cells" Materials 12, no. 12: 2016. https://doi.org/10.3390/ma12122016