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Materials 2018, 11(8), 1465; https://doi.org/10.3390/ma11081465

Biological Compatibility of a Polylactic Acid Composite Reinforced with Natural Chitosan Obtained from Shrimp Waste

1
Department of Metallurgical and Materials Engineering, Escuela Superior de Ingeniería Química e Industrias Extractivas (ESIQIE), Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos (UPALM), Av. Instituto Politécnico Nacional S/N, C.P., Ciudad de México 07738, Mexico
2
Escuela Nacional de Ciencias Biológicas (ENCB), Department of Microbiology, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala S/N, Casco de Santo Tomás. C.P., Ciudad de México 11340, Mexico
3
Department of Materials, Universidad Autónoma Metropolitana-Azcapotzalco, San Pablo No.180, Col. Reynosa-Tamaulipas, C.P., Ciudad de México 02200, Mexico
*
Author to whom correspondence should be addressed.
Received: 14 May 2018 / Revised: 1 June 2018 / Accepted: 1 June 2018 / Published: 18 August 2018
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Abstract

The aim of this work is to evaluate the effect of chitosan content (1, 3 and 5 wt %) dispersed in polylactic acid (PLA) on the structure and properties of composites. Also, the hydrolytic degradation, and the cell viability and adhesion of human MG-63 osteoblasts are analyzed to determine the composites’ suitability for use in tissue engineering. For the manufacture of the materials, natural chitosan was extracted chemically from shrimp exoskeleton. The composites were fabricated by extrusion, because it is a low-cost process, it is reproducible, and it does not compromise the biocompatibility of the materials. FT-IR and XRD show that the chitosan does not change the polymer structure, and interactions between the composite components are discarded. In vitro degradation tests show that the composites do not induce significant pH changes in phosphate buffer solution due to their low susceptibility to hydrolytic degradation. The adhesion and morphological characteristics of the osteoblasts are evaluated using confocal microscopy and scanning electron microscopy. The cell viability is determined by the MTT assay. Osteoblasts adhesion is observed on the surface of PLA and composites. A higher amount of chitosan, higher number of cells with osteoblastic morphology, and mineralized nodules are observed on the composite surface. The highest metabolic activity is evidenced at 21 days. The results suggest that the Polylactic acid/chitosan composites are potentially suitable for use as a biomaterial. View Full-Text
Keywords: biocomposites; polylactic acid; chitosan; osteoblasts; cellular viability; biomineralization biocomposites; polylactic acid; chitosan; osteoblasts; cellular viability; biomineralization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Torres-Hernández, Y.G.; Ortega-Díaz, G.M.; Téllez-Jurado, L.; Castrejón-Jiménez, N.S.; Altamirano-Torres, A.; García-Pérez, B.E.; Balmori-Ramírez, H. Biological Compatibility of a Polylactic Acid Composite Reinforced with Natural Chitosan Obtained from Shrimp Waste. Materials 2018, 11, 1465.

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