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Materials 2017, 10(8), 973; https://doi.org/10.3390/ma10080973

Genetically Engineered Phage Induced Selective H9c2 Cardiomyocytes Patterning in PDMS Microgrooves

1
Korea Institute of Science and Technology Europe (KIST-Europe) Forschungsgesellschaft mbH, Campus E 7 1, 66123 Saarbrücken, Germany
2
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Korea
3
Department of Biomedical Engineering, University of Science and Technology (UST), Daejeon 34113, Korea
*
Author to whom correspondence should be addressed.
Received: 7 July 2017 / Revised: 1 August 2017 / Accepted: 9 August 2017 / Published: 21 August 2017
(This article belongs to the Special Issue Constitutive Modelling of Biological Tissues and Biomaterials)
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Abstract

A micro-patterned cell adhesive surface was prepared for future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro-patterns were prepared by a photolithography process. Afterwards, recombinant filamentous phages that displayed a short binding motif with a cell adhesive peptide (-RGD-) on p8 proteins were immobilized on PDMS microgrooves through simple contact printing to study the cellular response of rat H9c2 cardiomyocyte. While the cell density decreased on PDMS micro-patterns, we observed enhanced cell proliferation and cell to surface interaction on the RGD-phage coated PDMS microgrooves. The RGD-phage coating also supported a better alignment of cell spreading rather than isotropic cell growths as we observed on non-pattered PDMS surface. View Full-Text
Keywords: polydimethylsiloxane (PDMS); micro-patterns; RGD-phage; cell-surface interaction; rat H9c2 cardiomyocytes polydimethylsiloxane (PDMS); micro-patterns; RGD-phage; cell-surface interaction; rat H9c2 cardiomyocytes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Kim, Y.; Kwon, C.; Jeon, H. Genetically Engineered Phage Induced Selective H9c2 Cardiomyocytes Patterning in PDMS Microgrooves. Materials 2017, 10, 973.

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