Next Article in Journal
From Stochastic Foam to Designed Structure: Balancing Cost and Performance of Cellular Metals
Next Article in Special Issue
Smart Carriers and Nanohealers: A Nanomedical Insight on Natural Polymers
Previous Article in Journal
Failure Behavior of Unidirectional Composites under Compression Loading: Effect of Fiber Waviness
Previous Article in Special Issue
Metallic Biomaterials: Current Challenges and Opportunities
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Materials 2017, 10(8), 910; doi:10.3390/ma10080910

Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration

4,* and 1,5,*
Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan
Department of Orthopedics, Show-Chwan Memorial Hospital, Changhua 50544, Taiwan
Department of Orthopedic Surgery, Faculty of Medicine, National Yang-Ming University, Taipei 112, Taiwan
Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung 40724, Taiwan
Medical Device Innovation Center, National Cheng Kung University, Tainan 701, Taiwan
Ming-Hsien Hu and Pei-Yuan Lee had equal contribution to this study.
Authors to whom correspondence should be addressed.
Received: 26 June 2017 / Revised: 19 July 2017 / Accepted: 3 August 2017 / Published: 6 August 2017
(This article belongs to the Special Issue Biocompatibility of Materials)
View Full-Text   |   Download PDF [5970 KB, uploaded 6 August 2017]   |  


In this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during the preparation of the DCP-rich CPC. In vitro cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity were evaluated using progenitor bone cells. Bone graft performance was evaluated via a rat posterolateral lumbar fusion model and osteointegration of the implant. New bone formations in the restorative sites were assessed by micro-computed tomography (micro-CT) and histological analysis. We found that the incorporation of collagen into the DCP-rich CPC was associated with increased cell adhesion, cell viability, and ALP activity in vitro. The spinal fusion model revealed a significant increase in bone regeneration. Additionally, better osseointegration was observed between the host bone and graft with the DCP-rich CPC supplemented with collagen than with the collagen-free DCP-rich CPC control graft. Furthermore, compared to the control graft, the results of micro-CT showed that a smaller amount of residual material was observed with the collagen-containing DCP-rich CPC graft compared with the control graft, which suggests the collagen supplement enhanced new bone formation. Of the different mixtures evaluated in this study (0.8 g DCP-rich CPC supplemented with 0.1, 0.2, and 0.4 mL type I collagen, respectively), DCP-rich CPC supplemented with 0.4 mL collagen led to the highest level of osteogenesis. Our results suggest that the DCP-rich CPC supplemented with collagen has potential to be used as an effective bone graft material in spinal surgery. View Full-Text
Keywords: posterolateral lumbar fusion; non-dispersive calcium phosphate bone cement; collagen; osteogenesis posterolateral lumbar fusion; non-dispersive calcium phosphate bone cement; collagen; osteogenesis

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hu, M.-H.; Lee, P.-Y.; Chen, W.-C.; Hu, J.-J. Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration. Materials 2017, 10, 910.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top