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Materials 2017, 10(7), 804; doi:10.3390/ma10070804

Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix Parvicella In Situ

1
School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China
2
Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China
3
School of Science, Tianjin Chengjian University, Tianjin 300384, China
*
Author to whom correspondence should be addressed.
Received: 8 June 2017 / Revised: 1 July 2017 / Accepted: 7 July 2017 / Published: 15 July 2017
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Abstract

In this study, two novel fluorescent probes, probe A and probe B were designed, synthesized and characterized, based on Microthrix parvicella (M. parvicella) preferring to utilize long-chain fatty acid (LCFA), for the labeling of M. parvicella in activated sludge. The molecular structure of probe A and probe B include long-chain alkane and LCFA, respectively. The results indicated that probe A and probe B had a large stokes shift of 118 nm and 120 nm and high quantum yield of 0.1043 and 0.1058, respectively, which were significantly helpful for the fluorescent labeling. As probe A was more stable than probe B in activated sludge, and the fluorescence intensity keep stable during 24 h, probe A was more suitable for labeling M. parvicella in situ. In addition, through the Image Pro Plus 6 (IPP 6) analysis, a quantitative relationship was established between sludge volume index (SVI) and integral optical density (IOD) of the labeled M. parvicella in activated sludge samples. The relationship between IOD and SVI conforms to Logistic curve (R2 = 0.94). View Full-Text
Keywords: fluorescent probes; sludge bulking; identification; Microthrix parvicella; quantitative analysis fluorescent probes; sludge bulking; identification; Microthrix parvicella; quantitative analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Jiao, X.; Fei, X.; Li, S.; Lin, D.; Ma, H.; Zhang, B. Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix Parvicella In Situ. Materials 2017, 10, 804.

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