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Materials 2017, 10(6), 568; doi:10.3390/ma10060568

Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration

Department of Materials Science and Metallurgy, University of Cambridge, Cambridge CB3 0FS, UK
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Academic Editor: Elisa Mele
Received: 20 March 2017 / Revised: 10 May 2017 / Accepted: 11 May 2017 / Published: 23 May 2017
(This article belongs to the Special Issue Naturally-Derived Biomaterials and Biopolymers)
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Abstract

A significant body of research has considered collagen as a scaffold material for soft tissue regeneration. The main structural component of extra-cellular matrix (ECM), collagen’s advantages over synthetic polymers are numerous. However, for applications where higher stiffness and stability are required, significant cross-linking may affect bioactivity. A carbodiimide (EDC) cross-linking route consumes carboxylate groups that are key to collagen’s essential cell recognition motifs (GxOGER). Fibrinogen was considered as a promising additive as it plays a key role in the process of wound repair and contains RGD integrin binding sites which bind to a variety of cells, growth factors and cytokines. Fibrinogen’s binding sites however, also contain the same carboxylate groups as collagen. We have successfully produced highly interconnected, porous collagen-fibrinogen scaffolds using a lyophilisation technique and micro-computed tomography demonstrated minimal influence of either fibrinogen content or cross-linking concentration on the scaffold structure. The specific biological effect of fibrinogen additions into cross-linked collagen are considered by using films as a model for the struts of bulk scaffolds. By considering various additions of fibrinogen to the collagen film with increasing degrees of cross-linking, this study demonstrates a significant biological advantage with fibrinogen addition across the cross-linking concentrations typically applied to collagen-based scaffolds. View Full-Text
Keywords: collagen; fibrinogen; adhesion; lyophilisation; micro-computed tomography collagen; fibrinogen; adhesion; lyophilisation; micro-computed tomography
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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  • Externally hosted supplementary file 1
    Doi: https://doi.org/10.17863/CAM.9603
    Description: Additional data related to this publication is available at:

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MDPI and ACS Style

Shepherd, J.; Bax, D.; Best, S.; Cameron, R. Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration. Materials 2017, 10, 568.

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