Effect of Immobilized Antithrombin III on the Thromboresistance of Polycarbonate Urethane
AbstractThe surface of foils and vascular grafts made from a thermoplastic polycarbonate urethanes (PCU) (Chronoflex AR) were chemically modified using gas plasma treatment, binding of hydrogels—(1) polyethylene glycol bisdiamine and carboxymethyl dextran (PEG-DEX) and (2) polyethyleneimine (PEI)—and immobilization of human antithrombin III (AT). Their biological impact was tested in vitro under static and dynamic conditions. Static test methods showed a significantly reduced adhesion of endothelial cells, platelets, and bacteria, compared to untreated PCU. Modified PCU grafts were circulated in a Chandler-Loop model for 90 min at 37 °C with human blood. Before and after circulation, parameters of the hemostatic system (coagulation, platelets, complement, and leukocyte activation) were analyzed. PEI-AT significantly inhibited the activation of both coagulation and platelets and prevented the activation of leukocytes and complement. In conclusion, both modifications significantly reduce coagulation activation, but only PEI-AT creates anti-bacterial and anti-thrombogenic functionality. View Full-Text
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Lukas, K.; Stadtherr, K.; Gessner, A.; Wehner, D.; Schmid, T.; Wendel, H.P.; Schmid, C.; Lehle, K. Effect of Immobilized Antithrombin III on the Thromboresistance of Polycarbonate Urethane. Materials 2017, 10, 335.
Lukas K, Stadtherr K, Gessner A, Wehner D, Schmid T, Wendel HP, Schmid C, Lehle K. Effect of Immobilized Antithrombin III on the Thromboresistance of Polycarbonate Urethane. Materials. 2017; 10(4):335.Chicago/Turabian Style
Lukas, Karin; Stadtherr, Karin; Gessner, Andre; Wehner, Daniel; Schmid, Thomas; Wendel, Hans P.; Schmid, Christof; Lehle, Karla. 2017. "Effect of Immobilized Antithrombin III on the Thromboresistance of Polycarbonate Urethane." Materials 10, no. 4: 335.
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