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Materials 2017, 10(3), 288; doi:10.3390/ma10030288

Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods

1
Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, Korea
2
Brain Korea 21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Korea
3
Dental Device Testing and Evaluation Center, Yonsei University College of Dentistry, Seoul 03722, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Mohan Jacob
Received: 16 January 2017 / Revised: 6 March 2017 / Accepted: 9 March 2017 / Published: 14 March 2017
(This article belongs to the Special Issue Biocompatibility of Materials)
View Full-Text   |   Download PDF [4747 KB, uploaded 14 March 2017]   |  

Abstract

Dental light-cured resins can undergo different degrees of polymerization when applied in vivo. When polymerization is incomplete, toxic monomers may be released into the oral cavity. The present study assessed the cytotoxicity of different materials, using sample preparation methods that mirror clinical conditions. Composite and bonding resins were used and divided into four groups according to sample preparation method: uncured; directly cured samples, which were cured after being placed on solidified agar; post-cured samples were polymerized before being placed on agar; and “removed unreacted layer” samples had their oxygen-inhibition layer removed after polymerization. Cytotoxicity was evaluated using an agar diffusion test, MTT assay, and confocal microscopy. Uncured samples were the most cytotoxic, while removed unreacted layer samples were the least cytotoxic (p < 0.05). In the MTT assay, cell viability increased significantly in every group as the concentration of the extracts decreased (p < 0.05). Extracts from post-cured and removed unreacted layer samples of bonding resin were less toxic than post-cured and removed unreacted layer samples of composite resin. Removal of the oxygen-inhibition layer resulted in the lowest cytotoxicity. Clinicians should remove unreacted monomers on the resin surface immediately after restoring teeth with light-curing resin to improve the restoration biocompatibility. View Full-Text
Keywords: biocompatibility; cytotoxicity; oxygen-inhibition layer; sample preparation; resin-based materials biocompatibility; cytotoxicity; oxygen-inhibition layer; sample preparation; resin-based materials
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lee, M.-J.; Kim, M.-J.; Kwon, J.-S.; Lee, S.-B.; Kim, K.-M. Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods. Materials 2017, 10, 288.

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