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Energies 2016, 9(4), 253; doi:10.3390/en9040253

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

1
Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
2
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
3
Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
4
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
5
Fermantation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand
*
Author to whom correspondence should be addressed.
Academic Editor: Tariq Al-Shemmeri
Received: 21 February 2016 / Revised: 12 March 2016 / Accepted: 15 March 2016 / Published: 31 March 2016
(This article belongs to the Special Issue Advances in Biomass for Energy Technology)
View Full-Text   |   Download PDF [1338 KB, uploaded 31 March 2016]   |  

Abstract

Ethanol production at elevated temperatures requires high potential thermotolerant ethanol-producing yeast. In this study, nine isolates of thermotolerant yeasts capable of growth and ethanol production at high temperatures were successfully isolated. Among these isolates, the newly isolated thermotolerant yeast strain, which was designated as Saccharomyces cerevisiae DBKKU Y-53, exhibited great potential for ethanol production from sweet sorghum juice (SSJ) at high temperatures. The maximum ethanol concentrations produced by this newly isolated thermotolerant yeast at 37 °C and 40 °C under the optimum cultural condition were 106.82 g·L−1 and 85.01 g·L−1, respectively, which are greater than values reported in the literatures. It should be noted from this study with SSJ at a sugar concentration of 250 g·L−1 and an initial pH of 5.5 without nitrogen supplementation can be used directly as substrate for ethanol production at high temperatures by thermotolerant yeast S. cerevisiae DBKKU Y-53. Gene expression analysis using real-time RT-PCR clearly indicated that growth and ethanol fermentation activities of the thermotolerant yeast S. cerevisiae DBKKU Y-53 at a high temperature (40 °C) were not only restricted to the expression of genes involved in the heat-shock response, but also to those genes involved in ATP production, trehalose and glycogen metabolism, and protein degradation processes were also involved. View Full-Text
Keywords: high temperature fermentation; real-time RT-PCR; Saccharomyces cerevisiae; sweet sorghum; thermotolerant yeast; gene expression high temperature fermentation; real-time RT-PCR; Saccharomyces cerevisiae; sweet sorghum; thermotolerant yeast; gene expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Nuanpeng, S.; Thanonkeo, S.; Yamada, M.; Thanonkeo, P. Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53. Energies 2016, 9, 253.

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