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Energies 2012, 5(9), 3178-3197; doi:10.3390/en5093178

Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design

1
Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
2
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
3
Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand
4
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
*
Author to whom correspondence should be addressed.
Received: 25 June 2012 / Revised: 1 August 2012 / Accepted: 17 August 2012 / Published: 24 August 2012
(This article belongs to the Special Issue Biomass and Biofuels 2012)
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Abstract

Optimization of four parameters, i.e., zinc (Zn2+), magnesium (Mg2+), manganese (Mn2+) and yeast extract for bioethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP 01 under very high gravity (VHG, 270 g·L−1 of total sugar) conditions was performed using an L9 (34) orthogonal array design. The fermentation was carried out at 30 °C in 500-mL air-locked Erlenmeyer flasks at the agitation rate of 100 rpm and the initial yeast cell concentration in the juice was approximately 5 × 107 cells·mL−1. The results showed that the order of influence was yeast extract > Mn2+ > Zn2+ > Mg2+ and the optimum nutrient concentrations for the ethanol fermentation were Zn2+, 0.01; Mg2+, 0.05; Mn2+, 0.04; and yeast extract, 9 g·L−1. The verification experiments under the optimum condition clearly indicated that the metals and nitrogen supplementation improved ethanol production efficiency under the VHG fermentation conditions. The ethanol concentration (P), yield (Yp/s) and productivity (Qp) were 120.58 ± 0.26 g·L−1, 0.49 ± 0.01 and 2.51 ± 0.01 g·L−1·h−1, respectively, while in the control treatment (without nutrient supplement) P, Yp/s and Qp were only 93.45 ± 0.45 g·L−1, 0.49 ± 0.00 and 1.30 ± 0.01 g·L−1·h−1, respectively. View Full-Text
Keywords: bioethanol; trace elements; nitrogen source; orthogonal array design; Saccharomyces cerevisiae; sweet sorghum juice; very high gravity (VHG) fermentation bioethanol; trace elements; nitrogen source; orthogonal array design; Saccharomyces cerevisiae; sweet sorghum juice; very high gravity (VHG) fermentation
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MDPI and ACS Style

Deesuth, O.; Laopaiboon, P.; Jaisil, P.; Laopaiboon, L. Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design. Energies 2012, 5, 3178-3197.

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