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Improving Production of Bioethanol from Duckweed (Landoltia punctata) by Pectinase Pretreatment
Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China
Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
* Author to whom correspondence should be addressed.
Received: 21 June 2012; in revised form: 18 July 2012 / Accepted: 6 August 2012 / Published: 10 August 2012
Abstract: Landoltia punctata, a widely distributed duckweed strain with the ability to accumulate starch, was used as a novel feedstock for bioethanol production by Saccharomyces cerevisiae. To improve ethanol production, pectinase pretreatment was used to release much more glucose from L. punctata mash and the pretreatment conditions (enzyme loading, temperature and pretreatment time) for the duckweed were optimized by using a surface response design. The results showed that maximum glucose yield was 218.64 ± 3.10 mg/g dry matter, which is a 142% increase compared to the untreated mash, with a pectinase dose of 26.54 pectin transeliminase unit/g mash at 45 °C for 300 min. Pectinase pretreatment apparently changed the ultrastructure of L. punctata, as evidenced by scanning electron microscopy analysis. Further fermentation experiments were performed and 30.8 ± 0.8 g/L of ethanol concentration, 90.04% of fermentation efficiency and 2.20 g/L/h of productivity rate were achieved. This is the highest ethanol concentration reported to date using duckweed as the feedstock.
Keywords: duckweed; Landoltia punctata; pectinase; ethanol fermentation; surface response design; Saccharomyces cerevisiae
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MDPI and ACS Style
Chen, Q.; Jin, Y.; Zhang, G.; Fang, Y.; Xiao, Y.; Zhao, H. Improving Production of Bioethanol from Duckweed (Landoltia punctata) by Pectinase Pretreatment. Energies 2012, 5, 3019-3032.
Chen Q, Jin Y, Zhang G, Fang Y, Xiao Y, Zhao H. Improving Production of Bioethanol from Duckweed (Landoltia punctata) by Pectinase Pretreatment. Energies. 2012; 5(8):3019-3032.
Chen, Qian; Jin, Yanling; Zhang, Guohua; Fang, Yang; Xiao, Yao; Zhao, Hai. 2012. "Improving Production of Bioethanol from Duckweed (Landoltia punctata) by Pectinase Pretreatment." Energies 5, no. 8: 3019-3032.