Next Article in Journal
Power Control Design for Variable-Speed Wind Turbines
Previous Article in Journal
Exploring Malaysia’s Transformation to Net Oil Importer and Oil Import Dependence
Article Menu

Export Article

Open AccessArticle
Energies 2012, 5(8), 3019-3032; doi:10.3390/en5083019

Improving Production of Bioethanol from Duckweed (Landoltia punctata) by Pectinase Pretreatment

1
Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu 610041, China
2
Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Received: 21 June 2012 / Revised: 18 July 2012 / Accepted: 6 August 2012 / Published: 10 August 2012
View Full-Text   |   Download PDF [734 KB, uploaded 17 March 2015]   |  

Abstract

Landoltia punctata, a widely distributed duckweed strain with the ability to accumulate starch, was used as a novel feedstock for bioethanol production by Saccharomyces cerevisiae. To improve ethanol production, pectinase pretreatment was used to release much more glucose from L. punctata mash and the pretreatment conditions (enzyme loading, temperature and pretreatment time) for the duckweed were optimized by using a surface response design. The results showed that maximum glucose yield was 218.64 ± 3.10 mg/g dry matter, which is a 142% increase compared to the untreated mash, with a pectinase dose of 26.54 pectin transeliminase unit/g mash at 45 °C for 300 min. Pectinase pretreatment apparently changed the ultrastructure of L. punctata, as evidenced by scanning electron microscopy analysis. Further fermentation experiments were performed and 30.8 ± 0.8 g/L of ethanol concentration, 90.04% of fermentation efficiency and 2.20 g/L/h of productivity rate were achieved. This is the highest ethanol concentration reported to date using duckweed as the feedstock. View Full-Text
Keywords: duckweed; Landoltia punctata; pectinase; ethanol fermentation; surface response design; Saccharomyces cerevisiae duckweed; Landoltia punctata; pectinase; ethanol fermentation; surface response design; Saccharomyces cerevisiae
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chen, Q.; Jin, Y.; Zhang, G.; Fang, Y.; Xiao, Y.; Zhao, H. Improving Production of Bioethanol from Duckweed (Landoltia punctata) by Pectinase Pretreatment. Energies 2012, 5, 3019-3032.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Energies EISSN 1996-1073 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top