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Energies 2012, 5(4), 1215-1228; doi:10.3390/en5041215

Repeated-Batch Ethanol Production from Sweet Sorghum Juice by Saccharomyces cerevisiae Immobilized on Sweet Sorghum Stalks

1
Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
2
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
3
Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand
4
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
*
Author to whom correspondence should be addressed.
Received: 4 January 2012 / Revised: 5 April 2012 / Accepted: 12 April 2012 / Published: 23 April 2012
(This article belongs to the Special Issue Biomass and Biofuels 2012)
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Abstract

Sweet sorghum stalks were used as a low cost carrier for immobilization of Saccharomyces cerevisiae NP 01 to produce ethanol from sweet sorghum juice. The effects on ethanol production of carrier size (6 × 6 × 6 to 20 × 20 × 20 mm3) and initial cell concentrations (5 × 107 to 2 × 108 cells mL−1) for cell immobilization were investigated. The ethanol production medium was the juice containing 230 g L−1 of total sugar without nutrient supplementation. The fermentations were carried out under static conditions in 500-mL air-locked Erlenmeyer flasks at 30 °C. The results showed that the optimum size of sorghum stalk pieces for repeated-batch ethanol production was 6 × 6 × 6 mm3, while the optimum initial cell concentration for the immobilization was 1.0 × 108 cells mL−1. The immobilized yeast under these conditions could be used for at least eight successive batches without any losses of ethanol production efficiencies. The average ethanol concentration, productivity and yield of the eight successive batches were 99.28 ± 3.53 g L−1, 1.36 ± 0.05 g L−1 h−1 and 0.47 ± 0.03 g g−1, respectively. View Full-Text
Keywords: ethanol fermentation; immobilization; repeated-batch; sweet sorghum; Saccharomyces cerevisiae ethanol fermentation; immobilization; repeated-batch; sweet sorghum; Saccharomyces cerevisiae
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ariyajaroenwong, P.; Laopaiboon, P.; Jaisil, P.; Laopaiboon, L. Repeated-Batch Ethanol Production from Sweet Sorghum Juice by Saccharomyces cerevisiae Immobilized on Sweet Sorghum Stalks. Energies 2012, 5, 1215-1228.

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