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Energies 2017, 10(10), 1671; doi:10.3390/en10101671

Laser Radiation Induces Growth and Lipid Accumulation in the Seawater Microalga Chlorella pacifica

School of Life Sciences, Shandong University of Technology, Zibo 255049, China
These authors equally contributed to this work.
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Academic Editor: Paul L. Chen
Received: 13 September 2017 / Revised: 13 October 2017 / Accepted: 16 October 2017 / Published: 22 October 2017
(This article belongs to the Section Energy Sources)
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Abstract

The impacts of laser radiation (Nd: YAG laser, 1064 nm at 800 mW, He–Ne laser 808 nm at 6 W, semiconductor laser 632.8 nm at 40 mW) on growth and lipid accumulation of Chlorella pacifica were investigated in this study. The results showed growth rates increased 1.23, 1.41, and 1.40-fold over controls by 4 min Nd: YAG, 4 min He–Ne, and 8 min semiconductor laser treatments, respectively, whereas the corresponding nitrate reductase observed increased 1.25, 1.63, and 2.08-fold over controls. Moreover, total chlorophyll concentration was increased to 1.09, 1.29, and 1.33-fold over controls, respectively. After 20 days cultivation, the highest lipid content was 35.99%, 18.46%, and 31.00% after 2 min Nd: YAG, 4 min He–Ne, and 4 min semiconductor laser treatments, corresponding to 2.86, 1.50, and 2.46-fold increase over controls, respectively. Furthermore, the lipid productivity of the above 3 treatments were 15.25 ± 2.56, 16.25 ± 2.45, and 14.75 ± 2.11 mg L−1 d−1. However, the highest lipid productivity was 22.00 ± 3.28, 16.25 ± 2.45, and 19.25 ± 1.78 mg L−1 d−1, in response to treatment for 2 min Nd: YAG, 1 min He–Ne, and 4 min semiconductor laser treatments, with 2.67, 1.97, and 2.33-fold increase over controls, respectively. These results indicated that lipid accumulation efficiency of C. pacifica could be significantly improved by laser irradiation using Nd: YAG, He–Ne, and semiconductor laser treatments. View Full-Text
Keywords: cell growth; Chlorella pacifica; chlorophylls; laser irradiation; lipid productivity; nitrate reductase cell growth; Chlorella pacifica; chlorophylls; laser irradiation; lipid productivity; nitrate reductase
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Zhang, H.; Gao, Z.; Li, Z.; Du, H.; Lin, B.; Cui, M.; Yin, Y.; Lei, F.; Yu, C.; Meng, C. Laser Radiation Induces Growth and Lipid Accumulation in the Seawater Microalga Chlorella pacifica. Energies 2017, 10, 1671.

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