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Oxidative Response and Antioxidative Mechanism in Germinating Soybean Seeds Exposed to Cadmium
Section of Ecology, School of Life Sciences, Anhui Normal University, Wuhu 241000, China
School of Environmental Science and Engineering, Anhui Normal University, Wuhu 241000, China
* Author to whom correspondence should be addressed.
Received: 14 June 2012; in revised form: 4 July 2012 / Accepted: 31 July 2012 / Published: 8 August 2012
Abstract: Seeds of soybean (Glycine max L.) exposed to 50 mg/L (Cd50), 100 mg/L (Cd100) and 200 mg/L (Cd200) cadmium solution for 24, 48, 72 and 96 h were examined with reference to Cd accumulation, oxidative stress and antioxidative responses. Soybean seeds accumulated Cd in an exposure time-and dosage-dependent manner. FRAP (ferric reducing ability of plasma) concentration, GSH/hGSH content, and GST activity showed a pronounced exposure time-dependent response. Cd100 enhanced FRAP concentration in germinating soybean seeds as compared to Cd50 treatment after 24 h exposure. Cd200 however increased statistically GST activities after 72 and 96 h exposure. Under all Cd dosages, GSH/hGSH concentrations were depressed with increasing exposure time. Reduction of GSH/hGSH content and concomitant increase of GST activity suggested a possible participation of GSH into GSH-Cd conjugates synthesis. MDA content is a potential biomarker for monitoring Cd phytotoxicity because it responds significantly to treatment dosage, exposure time and dosage ´ exposure time interaction. Increase of proline content may be a response to acute heavy metal toxicity in soybean seeds.
Keywords: cadmium; soybean; oxidative stress; antioxidative response; bioindicator
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MDPI and ACS Style
Yang, S.; Xie, J.; Li, Q. Oxidative Response and Antioxidative Mechanism in Germinating Soybean Seeds Exposed to Cadmium. Int. J. Environ. Res. Public Health 2012, 9, 2827-2838.
Yang S, Xie J, Li Q. Oxidative Response and Antioxidative Mechanism in Germinating Soybean Seeds Exposed to Cadmium. International Journal of Environmental Research and Public Health. 2012; 9(8):2827-2838.
Yang, Shiyong; Xie, Jianchun; Li, Quanfa. 2012. "Oxidative Response and Antioxidative Mechanism in Germinating Soybean Seeds Exposed to Cadmium." Int. J. Environ. Res. Public Health 9, no. 8: 2827-2838.