Next Article in Journal
Human Arsenic Poisoning Issues in Central-East Indian Locations: Biomarkers and Biochemical Monitoring
Previous Article in Journal
Toxicity and Carcinogenicity Mechanisms of Fibrous Antigorite
Article Menu

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2007, 4(1), 10-14; doi:10.3390/ijerph2007010002

Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

1
Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA
2
College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA
3
Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA
*
Author to whom correspondence should be addressed.
Received: 31 July 2006 / Accepted: 21 February 2007 / Published: 31 March 2007
View Full-Text   |   Download PDF [155 KB, uploaded 19 June 2014]   |  

Abstract

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G1 phase, while about 76% were arrested at G1 phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells. View Full-Text
Keywords: Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Nwagbara, O.; Darling-Reed, S.F.; Tucker, A.; Harris, C.; Abazinge, M.; Thomas, R.D.; Gragg, R.D. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. Int. J. Environ. Res. Public Health 2007, 4, 10-14.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top