Next Article in Journal
Human Arsenic Poisoning Issues in Central-East Indian Locations: Biomarkers and Biochemical Monitoring
Previous Article in Journal
Toxicity and Carcinogenicity Mechanisms of Fibrous Antigorite
Int. J. Environ. Res. Public Health 2007, 4(1), 10-14; doi:10.3390/ijerph2007010002
Article

Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

1,* , 2, 2, 3, 1, 2 and 1
1 Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA 2 College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA 3 Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA
* Author to whom correspondence should be addressed.
Received: 31 July 2006 / Accepted: 21 February 2007 / Published: 31 March 2007
View Full-Text   |   Download PDF [155 KB, uploaded 19 June 2014]   |   Browse Figures
SciFeed

Abstract

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G1 phase, while about 76% were arrested at G1 phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells.
Keywords: Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Nwagbara, O.; Darling-Reed, S.F.; Tucker, A.; Harris, C.; Abazinge, M.; Thomas, R.D.; Gragg, R.D. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. Int. J. Environ. Res. Public Health 2007, 4, 10-14.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert