Int. J. Environ. Res. Public Health 2007, 4(1), 10-14; doi:10.3390/ijerph2007010002
Article

Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

1 Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA 2 College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA 3 Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA
* Author to whom correspondence should be addressed.
Received: 31 July 2006; Accepted: 21 February 2007 / Published: 31 March 2007
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Abstract: Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G1 phase, while about 76% were arrested at G1 phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells.
Keywords: Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle

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MDPI and ACS Style

Nwagbara, O.; Darling-Reed, S.F.; Tucker, A.; Harris, C.; Abazinge, M.; Thomas, R.D.; Gragg, R.D. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. Int. J. Environ. Res. Public Health 2007, 4, 10-14.

AMA Style

Nwagbara O, Darling-Reed SF, Tucker A, Harris C, Abazinge M, Thomas RD, Gragg RD. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. International Journal of Environmental Research and Public Health. 2007; 4(1):10-14.

Chicago/Turabian Style

Nwagbara, Onyinye; Darling-Reed, Selina F.; Tucker, Alicia; Harris, Cynthia; Abazinge, Michael; Thomas, Ronald D.; Gragg, Richard D. 2007. "Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide." Int. J. Environ. Res. Public Health 4, no. 1: 10-14.

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