Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

doi:10.3390/ijerph2007010002

This article is

freely available
re-usable

Int. J. Environ. Res. Public Health 2007, 4(1), 10-14; doi:10.3390/ijerph2007010002

Article

Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

Onyinye Nwagbara^1,* , Selina F. Darling-Reed², Alicia Tucker², Cynthia Harris³, Michael Abazinge¹, Ronald D. Thomas² and Richard D. Gragg¹

¹ Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA ² College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA ³ Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA

* Author to whom correspondence should be addressed.

Received: 31 July 2006 / Accepted: 21 February 2007 / Published: 31 March 2007

Download PDF Full-Text [155 KB, uploaded 16 September 2008 11:01 CEST]

Abstract: Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G1 phase, while about 76% were arrested at G1 phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells.

Keywords: Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Nwagbara, O.; Darling-Reed, S.F.; Tucker, A.; Harris, C.; Abazinge, M.; Thomas, R.D.; Gragg, R.D. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. Int. J. Environ. Res. Public Health 2007, 4, 10-14.

AMA Style

Nwagbara O., Darling-Reed S.F., Tucker A., Harris C., Abazinge M., Thomas R.D., Gragg R.D. Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide. International Journal of Environmental Research and Public Health. 2007; 4(1):10-14.

Chicago/Turabian Style

Nwagbara, Onyinye; Darling-Reed, Selina F.; Tucker, Alicia; Harris, Cynthia; Abazinge, Michael; Thomas, Ronald D.; Gragg, Richard D. 2007. "Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide." Int. J. Environ. Res. Public Health 4, no. 1: 10-14.

Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI Publishing, Basel, Switzerland RSS E-Mail Table of Contents Alert