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Int. J. Environ. Res. Public Health 2006, 3(1), 43-47; doi:10.3390/ijerph2006030006

The Effect of UV-B Radiation on Bufo arenarum Embryos Survival and Superoxide Dismutase Activity

* ,  and
Programa de Seguridad Química, Instituto de Ciencias Ambientales y Salud, Fundación PROSAMA, Paysandú 752 (1405), Buenos Aires, Argentina. Member of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) de la Argentina
* Author to whom correspondence should be addressed.
Received: 5 January 2006 / Accepted: 17 March 2006 / Published: 31 March 2006
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The exposure of Bufo arenarum embryos to 300-310 nm UV-B at a dose of 4,104 Joule/m2 resulted in 100% lethality within 24 hr while 820 Joule/m2 was the NOEC value for short-term chronic (10 days) exposure. The dose response curves show that lethal effects are proportional with the dose and achieve its highest value within 48 hr post exposure. The superoxide dismutase (SOD) activity in amphibian embryos for sublethal UV-B exposures was evaluated by means of UV-B treatments with 273 (A), 820(B), 1368(C) and 1915(D) Joule/m2 at 2 and 5 hours post irradiation. The SOD activity in units/mg protein in A, B, C and D at 2 hr after treatments were 80.72 ± 14.29, 74.5 ± 13.19, 39.5 ± 6.99 and 10.7 ± 1.89 respectively while for control embryos it was 10.88 ± 1.31. At 5 hr after treatments the SOD values were similar to those found in control embryos. The results confirm the high susceptibility of amphibian embryos to UV-B and point out that the SOD activity is enhanced by low doses of UV-B irradiation achieving significantly higher values than in control embryos at 2 hr post exposure.
Keywords: UV-B; Amphibian embryos; Superoxide dismutase UV-B; Amphibian embryos; Superoxide dismutase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Herkovits, J.; D´Eramo, J.L.; Fridman, O. The Effect of UV-B Radiation on Bufo arenarum Embryos Survival and Superoxide Dismutase Activity. Int. J. Environ. Res. Public Health 2006, 3, 43-47.

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