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Int. J. Environ. Res. Public Health 2017, 14(7), 676; doi:10.3390/ijerph14070676

Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Department of Microbiology and Parasitology, University of Navarra, 31080 Pamplona, Spain
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Academic Editor: Panagiotis Karanis
Received: 3 May 2017 / Revised: 5 June 2017 / Accepted: 19 June 2017 / Published: 23 June 2017
(This article belongs to the Section Environmental Health)
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Abstract

This study presents a comprehensive approach of a clonal diversity analysis of 448 Extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from environmental, human and food samples in Spain. The phenotypic confirmation of ESBL production was performed by disc diffusion and microdilution methods, while Polymerase Chain Reaction (PCR) and sequencing were used for the molecular characterization of β-lactamase genes (blaCTX-M, blaSHV, blaTEM, blaOXA). Clonal relationship of isolates was determined by multi-locus sequence typing (MLST). Multidrug resistant strains were present in all the studied niches, with percentages above 50.0%. The most prevalent β-lactamase genes were blaCTXM-14 (26%) and blaCTXM-1 (21.4%), followed by blaSHV-12, blaCTX-M-15 and blaTEM-42. MLST isolates were grouped into 26 clonal complexes (CC) and 177 different sequence types (ST) were detected. Despite the high clonal diversity observed, CC10 was the prevalent and the only CC detected in all niches, while other complexes as CC131 were mainly associated to human isolates. The observed prevalence and diversity of these resistant bacteria across the different environments encourages a One Health approach to prevent and control ESBL dissemination between environment and consumers. View Full-Text
Keywords: ESBL-producing E. coli; β-lactamase genes; clonal diversity; MLST ESBL-producing E. coli; β-lactamase genes; clonal diversity; MLST
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ojer-Usoz, E.; González, D.; Vitas, A.I. Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples. Int. J. Environ. Res. Public Health 2017, 14, 676.

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