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Int. J. Environ. Res. Public Health 2017, 14(6), 591; doi:10.3390/ijerph14060591

Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas

1
Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia
2
Department of Agroecology, Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia
*
Author to whom correspondence should be addressed.
Received: 7 April 2017 / Revised: 15 May 2017 / Accepted: 15 May 2017 / Published: 2 June 2017
(This article belongs to the Special Issue Nutrition and Public Health)
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Abstract

This study aimed to determine whether low dietary Se content affects the function and mechanisms mediating the vascular relaxation of rat aortas, and to test the role of oxidative stress in observed differences. Male Sprague Dawley (SD) rats were maintained for 10 weeks on low Se (low-Se group; N = 20) or normal Se content (norm-Se group; N = 20) rat chow. Dose responses to acetylcholine (ACh; 10−9–10−5M) and the response to reduced pO2 were tested in noradrenaline-precontracted aortic rings in the absence/presence of the nitric oxide synthase (NOS) inhibitor nitro-l-arginine methyl ester (l-NAME), the cyclooxygenase 1 and 2 (COX-1, 2) inhibitor Indomethacin, and the antioxidative agent Tempol in tissue bath. mRNA expression of glutathione peroxidase 1 (GPx1), catalase (CAT), and Cu/Zn superoxide dismutase (SOD) was measured in rat aortas. Oxidative stress (Thiobarbituric Acid Reactive Substances; TBARS), antioxidative plasma capacity (ferric reducing ability of plasma assay; FRAP), and protein levels of GPx1 were measured in plasma and serum samples, respectively. Reduced ACh-induced relaxation (AChIR) (dominantly mediated by NO) in the low-Se group compared to the norm-Se group was restored by Tempol administration. Hypoxia-induced relaxation (HIR) (dominantly mediated by COX-1, 2), TBARS, and FRAP as well as GPx1 serum concentrations were similar between the groups. mRNA GPx1 expression in rat aortas was significantly decreased in the low-Se compared to the norm-Se group. These data suggest that low dietary Se content increases the local oxidative stress level, which subsequently affects the NO-mediated vascular response. View Full-Text
Keywords: selenium; vascular function; endothelium; oxidative stress; rats selenium; vascular function; endothelium; oxidative stress; rats
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MDPI and ACS Style

Stupin, A.; Cosic, A.; Novak, S.; Vesel, M.; Jukic, I.; Popovic, B.; Karalic, K.; Loncaric, Z.; Drenjancevic, I. Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas. Int. J. Environ. Res. Public Health 2017, 14, 591.

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