Next Article in Journal
Urban Place and Health Equity: Critical Issues and Practices
Next Article in Special Issue
Development of a New Microextraction Fiber Combined to On-Line Sample Stacking Capillary Electrophoresis UV Detection for Acidic Drugs Determination in Real Water Samples
Previous Article in Journal
Racial Differences in Perceptions of Air Pollution Health Risk: Does Environmental Exposure Matter?
Previous Article in Special Issue
Polypyrrole-Grafted Coconut Shell Biological Carbon as a Potential Adsorbent for Methyl Tert-Butyl Ether Removal: Characterization and Adsorption Capability
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2017, 14(2), 115; doi:10.3390/ijerph14020115

Isolation, Characterization, and Degradation Performance of the 17β-Estradiol-Degrading Bacterium Novosphingobium sp. E2S

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
These authors contributed equally to this paper.
Authors to whom correspondence should be addressed.
Academic Editor: Emmanuelle Vulliet
Received: 4 November 2016 / Revised: 16 January 2017 / Accepted: 16 January 2017 / Published: 25 January 2017
(This article belongs to the Special Issue Organic Micro-Pollutants and Human Exposure)
View Full-Text   |   Download PDF [2265 KB, uploaded 25 January 2017]   |  


A 17β-estradiol (E2)-degrading bacterium E2S was isolated from the activated sludge in a sewage treatment plant (STP). The morphology, biological characteristics, and 16S ribosomal RNA (rRNA) gene sequence of strain E2S indicated that it belonged to the genus Novosphingobium. The optimal degrading conditions were 30 °C and pH 7.0. The ideal inoculum volume was 5% (v/v), and a 20-mL degradation system was sufficient to support the removal ability of strain E2S. The addition of extra NaCl to the system did not benefit the E2 degradation in batch culture by this strain. Strain E2S exhibited high degradation efficiency with initial substrate concentrations of 10–50 mg·L−1. For example, in mineral salt medium containing 50 mg·L−1 of E2, the degradation efficiency was 63.29% after seven days. In cow manure samples supplemented with 50 mg·L−1 of E2, strain E2S exhibited 66.40% degradation efficiency after seven days. The finding of the E2-degrading strain E2S provided a promising method for removing E2 from livestock manure in order to reduce the potential environmental risks of E2. View Full-Text
Keywords: 17β-estradiol; estrogen; degradation; Novosphingobium sp.; cow manure 17β-estradiol; estrogen; degradation; Novosphingobium sp.; cow manure

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, S.; Liu, J.; Sun, M.; Ling, W.; Zhu, X. Isolation, Characterization, and Degradation Performance of the 17β-Estradiol-Degrading Bacterium Novosphingobium sp. E2S. Int. J. Environ. Res. Public Health 2017, 14, 115.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top